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Amperometric enzyme sensor for the rapid determination of histamine

2019Journal article Open access
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dc.contributor.authorTorre, Ricarda
dc.contributor.authorCosta Rama, Estefanía
dc.contributor.authorLOPES, Paula
dc.contributor.authorNouws, Henri
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2020-07-10T14:39:20Z
dc.date.available2020-07-10T14:39:20Z
dc.date.issued2019
dc.description.abstractThe concentration of histamine, a biogenic amine, in fish is considered a fish spoilage indicator. Therefore, the development of a rapid-response and portable tool that allows its on-site analysis is very interesting since the consumption of high amounts of histamine causes scombroid poisoning. Thus, in this work a simple enzymatic sensor for the determination of histamine, based on a screen-printed carbon electrode and the enzyme diamine oxidase, was developed. The enzyme was immobilized on the electrode surface through a simple cross-linking procedure employing glutaraldehyde and bovine serum albumin. Chronoamperometry was used as the detection technique; the sensor showed a short response time (60 s, -0.3 V) and the measurements were performed by only using 40 μL of sample solution. The sensor provides an useful linear range (between 1 and 75 mg L-1 in fish extract), excellent reproducibility (RSD = 2.6%) and is reusable for up to 7 measurements. The feasibility of the sensor was tested performing histamine analysis in fish extracts achieving recovery values of 103%.pt_PT
dc.description.sponsorshipThe authors are grateful for the financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 through the project UID/ QUI/50006/2013. The authors would also like to thank the EU and FCT for funding through the projects: FishBioSensing - Portable electrochemical (bio)sensing devices for safety and quality assessment of fishery products (02/SAICT/2016, POCI-01-0145-FEDER-023817) and FOODnanoHEALTH - Qualidade e Segurança Alimentar – uma abordagem (nano)tecnológica (Portugal2020, Norte-01-0145-FEDER-000011).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1039/C8AY02610Fpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16106
dc.language.isoengpt_PT
dc.publisherRSCpt_PT
dc.relation.publisherversionhttps://pubs.rsc.org/en/content/articlelanding/2019/AY/C8AY02610F#!divAbstractpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectFishpt_PT
dc.subjectHistaminept_PT
dc.subjectScreen-printed carbon electrodept_PT
dc.subjectEnzymatic sensorpt_PT
dc.titleAmperometric enzyme sensor for the rapid determination of histaminept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.endPage1269pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1264pt_PT
oaire.citation.titleAnalytical Methodspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream5876
person.familyNameTorre
person.familyNameCosta Rama
person.familyNameLOPES
person.familyNameNouws
person.familyNameDelerue-Matos
person.givenNameRicarda
person.givenNameEstefanía
person.givenNamePaula
person.givenNameHendrikus
person.givenNameCristina
person.identifier1046746
person.identifier.ciencia-id2015-9883-AD97
person.identifier.ciencia-id3614-E403-0136
person.identifier.ciencia-id7C18-36BB-82B6
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person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0003-2843-8402
person.identifier.orcid0000-0002-5357-9269
person.identifier.orcid0000-0003-4034-7722
person.identifier.orcid0000-0002-1472-8866
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridJ-5815-2018
person.identifier.ridD-5249-2013
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id36703129800
person.identifier.scopus-author-id55808813100
person.identifier.scopus-author-id7801450247
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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