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Enhancement of wound healing in diabetic mice by topical use of a peptide-ionic liquid conjugate

2025-03Journal article Embargoed access
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dc.contributor.authorGomes, Ana
dc.contributor.authorLeal, Ermelindo C.
dc.contributor.authorFerraz, Ricardo
dc.contributor.authorCalheiros, Daniela
dc.contributor.authorSilva, Jessica da
dc.contributor.authorGonçalves, Teresa
dc.contributor.authorTeixeira, Inês
dc.contributor.authorCarvalho, Eugénia
dc.contributor.authorGomes, Paula
dc.contributor.authorFerraz, Ricardo
dc.date.accessioned2025-12-23T14:59:30Z
dc.date.available2025-12-23T14:59:30Z
dc.date.issued2025-03
dc.description.abstractDiabetic foot ulcers (DFU) are one of the most devastating complications of diabetes, with high impact on patient’s quality of life. In worst scenarios, DFU can lead to severe amputation or even death. DFUs are an easy target for microbial pathogens and their effective healing is hampered by the galloping increase of microbial resistance to antibiotics, including from the most prevalent pathogens in DFU, e.g. Staphylococcus aureus. As such, available antibiotics show poor efficacy in the treatment of DFU, leading to a chronic condition that is exacerbated by poor healing rates due to the persistent inflammation, poor oxygenation and low angiogenesis, leading to high risk of ischemia, among other conditions that typically affect patients with diabetes. Our group has recently designed new peptide-based strategies towards the topical treatment of DFU, whereby peptide-ionic liquid conjugates emerged as highly promising agents. One of the best such conjugates, C16-Im-PP4, results from coupling an imidazolium-based ionic liquid with intrinsic antimicrobial activity to the N-terminus of a collagen boosting peptide used in cosmetics, the pentapeptide-4. C16-Im-PP4 showed excellent in vitro properties, namely, wide-spectrum antimicrobial action and collagen-boosting effect on human dermal fibroblasts, prompting the in vivo study here reported. The peptide-ionic liquid conjugate was applied topically on wounds of mice with diabetes. The results show multitargeted actions, at a dose of 1 µg/wound including: i) anti-inflammatory; ii) antioxidant; iii) pro-collagenic; vi) pro-angiogenic; v) antimicrobial; and vi) improved wound maturation effects. Altogether, these results identify this technology as a novel topical treatment for DFU.eng
dc.identifier.citationGomes, A., Leal, E. C., Da Silva, J., Teixeira, I., Ferraz, R., Calheiros, D., Gonçalves, T., Carvalho, E., & Gomes, P. (2025). Enhancement of wound healing in diabetic mice by topical use of a peptide-ionic liquid conjugate. The International Journal of Biochemistry & Cell Biology, 180, 106753. https://doi.org/10.1016/j.biocel.2025.106753
dc.identifier.doi10.1016/j.biocel.2025.106753
dc.identifier.eissn1878-5875
dc.identifier.issn1357-2725
dc.identifier.urihttp://hdl.handle.net/10400.22/31346
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationUIDP/50006/2020
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S1357272525000202
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnti-inflammatory
dc.subjectAntioxidant
dc.subjectAntimicrobial peptide
dc.subjectDiabetic foot ulcers
dc.titleEnhancement of wound healing in diabetic mice by topical use of a peptide-ionic liquid conjugateeng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleThe International Journal of Biochemistry & Cell Biology
oaire.citation.volume180
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameFerraz
person.givenNameRicardo
person.identifier.ciencia-id001E-71CE-F92D
person.identifier.orcid0000-0002-1761-117X
person.identifier.ridG-5639-2011
person.identifier.scopus-author-id24464208500
relation.isAuthorOfPublicationa5a8faa7-12a5-4b1c-bced-44c895677397
relation.isAuthorOfPublication.latestForDiscoverya5a8faa7-12a5-4b1c-bced-44c895677397

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