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Authors
Advisor(s)
Abstract(s)
O trabalho consistiu no desenvolvimento e caracterização de sensores potenciométricos
com base em polímeros de impressão molecular para a determinação de um antibiótico, a
norfloxacina, em aquacultura.
A simplicidade, o baixo custo e a interação rápida e reversível dos sensores
potenciométricos com os analitos fizeram com que este fosse o tipo de sensor escolhido. O
material sensor foi obtido por tecnologia de impressão molecular, baseada em polimerização
em bulk, em que a NOR foi a molécula molde e foram utilizados como monómeros para
autoconstrução dos sensores o pirrol, isoladamente, ou em conjunto com partículas de sílica
gel funcionalizadas com 3-aminopropil. Também foi obtido material sensor, para controlo,
em que a molécula molde NOR não estava presente (NIP). As características dos materiais
sensores foram sujeitas a análise de microscopia eletrónica SEM e análise por
espectrómetro de infravermelhos com transformada de Fourier.
Os materiais sensores foram incluídos em membranas poliméricas, que seriam incorporadas
em elétrodos. A avaliação do desempenho dos elétrodos foi feita através de curvas de
calibração em diferentes meios (PBS, MES e HEPES). Também foi efetuada com sucesso a
análise da sensibilidade dos elétrodos em água dopada.
As diversas avaliações e análises efetuadas levaram a concluir que o MIP de pirrol com
aditivo aniónico, foi o material sensor testado que permitiu obter melhores propriedades de
resposta.
The proposed work concerns the development and characterization of potentiometric sensors based on molecularly imprinted polymers for the determination of norfloxacin, in aquaculture. The simplicity, low cost and rapid and reversible interaction of potentiometric sensors with the analytes led to choose this type of sensor. The sensor material was obtained by molecular imprinting technology based on bulk polymerization, wherein the NOR was the template molecule and were used as monomers the pyrrole, alone or together with silica gel particles functionalized with 3 -aminopropyl. The characteristics of the sensors materials were subjected to electron microscopy analysis SEM and analysis by infrared Fourier transform spectrometer. The sensors materials were included in polymer membranes which would be incorporated into electrodes. The evaluation of the performance of the electrodes was made using calibration curves in different buffers (PBS, MES and HEPES). It has also been successfully performed sensitivity analysis of doped electrodes in water. The various reviews and analyzes performed led to conclude that the MIP Py with anionic additive have the better response properties.
The proposed work concerns the development and characterization of potentiometric sensors based on molecularly imprinted polymers for the determination of norfloxacin, in aquaculture. The simplicity, low cost and rapid and reversible interaction of potentiometric sensors with the analytes led to choose this type of sensor. The sensor material was obtained by molecular imprinting technology based on bulk polymerization, wherein the NOR was the template molecule and were used as monomers the pyrrole, alone or together with silica gel particles functionalized with 3 -aminopropyl. The characteristics of the sensors materials were subjected to electron microscopy analysis SEM and analysis by infrared Fourier transform spectrometer. The sensors materials were included in polymer membranes which would be incorporated into electrodes. The evaluation of the performance of the electrodes was made using calibration curves in different buffers (PBS, MES and HEPES). It has also been successfully performed sensitivity analysis of doped electrodes in water. The various reviews and analyzes performed led to conclude that the MIP Py with anionic additive have the better response properties.
Description
Keywords
Sensor Norfloxacina Antibiótico Água Aquacultura Norfloxacin Antibiotic Water Aquaculture