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Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration

2016Journal article Restricted access
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dc.contributor.authorVasconcelos, Daniel M.
dc.contributor.authorGonçalves, Raquel M.
dc.contributor.authorAlmeida, Catarina R.
dc.contributor.authorPereira, Inês O.
dc.contributor.authorOliveira, Marta I.
dc.contributor.authorNeves, Nuno
dc.contributor.authorSilva, Andreia M.
dc.contributor.authorRibeiro, António C.
dc.contributor.authorCunha, Carla
dc.contributor.authorAlmeida, Ana R.
dc.contributor.authorRibeiro, Cristina C.
dc.contributor.authorGil, Ana M.
dc.contributor.authorSeebach, Elisabeth
dc.contributor.authorKynast, Katharina L.
dc.contributor.authorRichter, Wiltrud
dc.contributor.authorLamghari, Meriem
dc.contributor.authorSantos, Susana G.
dc.contributor.authorBarbosa, Mário A.
dc.date.accessioned2017-06-29T13:30:30Z
dc.date.embargo2117
dc.date.issued2016
dc.description.abstractThe hypothesis behind this work is that fibrinogen (Fg), classically considered a pro-inflammatory protein, can promote bone repair/regeneration. Injury and biomaterial implantation naturally lead to an inflammatory response, which should be under control, but not necessarily minimized. Herein, porous scaffolds entirely constituted of Fg (Fg-3D) were implanted in a femoral rat bone defect and investigated at two important time points, addressing the bone regenerative process and the local and systemic immune responses, both crucial to elucidate the mechanisms of tissue remodelling. Fg-3D led to early infiltration of granulation tissue (6 days post-implantation), followed by bone defect closure, including periosteum repair (8 weeks post-injury). In the acute inflammatory phase (6 days) local gene expression analysis revealed significant increases of pro-inflammatory cytokines IL-6 and IL-8, when compared with non-operated animals. This correlated with modified proportions of systemic immune cell populations, namely increased T cells and decreased B, NK and NKT lymphocytes and myeloid cell, including the Mac- 1þ (CD18þ/CD11bþ) subpopulation. At 8 weeks, Fg-3D led to decreased plasma levels of IL-1b and increased TGF-b1. Thus, our data supports the hypothesis, establishing a link between bone repair induced by Fg-3D and the immune response. In this sense, Fg-3D scaffolds may be considered immunomodulatory biomaterials.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.biomaterials.2016.10.004pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/9973
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0142961216305415pt_PT
dc.subjectFibrinogenpt_PT
dc.subjectIn vivopt_PT
dc.subjectBone repair/regenerationpt_PT
dc.subjectInflammationpt_PT
dc.subjectBiomaterialpt_PT
dc.titleFibrinogen scaffolds with immunomodulatory properties promote in vivo bone regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBiomaterialspt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT

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