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Advisor(s)
Abstract(s)
The characteristic topographical features (crystallite dimensions, surface morphology and roughness) of
bioceramics may influence the adsorption of proteins relevant to bone regeneration. This work aims at
analyzing the influence of two distinct nanophased hydroxyapatite (HA) ceramics, HA725 and HA1000
on fibronectin (FN) and osteonectin (ON) adsorption and MC3T3-E1 osteoblast adhesion and morphology.
Both substrates were obtained using the same hydroxyapatite nanocrystals aggregates and applying the
sintering temperatures of 725ºC and 1000ºC, respectively. The two proteins used in this work, FN as an
adhesive glycoprotein and ON as a counter-adhesive protein, are known to be involved in the early stages
of osteogenesis (cell adhesion, mobility and proliferation). The properties of the nanoHA substrates had
an important role in the adsorption behavior of the two studied proteins and clearly affected the MC3T3-
E1 morphology, distribution and metabolic activity. HA1000 surfaces presenting slightly larger grain size,
higher root-mean-square roughness (Rq), lower surface area and porosity, allowed for higher amounts of
both proteins adsorbed. These substrates also revealed increased number of exposed FN cell-binding
domains as well as higher affinity for osteonectin. Regarding the osteoblast adhesion results, improved
viability and cell number were found for HA1000 surfaces as compared to HA725 ones, independently
of the presence or type of adsorbed protein. Therefore the osteoblast adhesion and metabolic activity
seemed to be more sensitive to surfaces morphology and roughness than to the type of adsorbed proteins.
Description
Keywords
Biomaterials Nanophased hydroxyapatite Fibronectin adsorption Osteonectin adsorption Osteoblast adhesion Osteoblast morphology
Citation
Publisher
Elsevier