Repository logo
 
Publication

Screening of fluoroquinolones in environmental waters using disk-based solid-phase extraction combined to microplate fluorimetric determination and LC-MS/MS

2019Journal article Open access
Simple item page
dc.contributor.authorPeixoto, Patrícia S.
dc.contributor.authorTóth, Ildikó V.
dc.contributor.authorBarreiros, Luisa
dc.contributor.authorMachado, Ana
dc.contributor.authorBordalo, Adriano A.
dc.contributor.authorLima, José L. F. C.
dc.contributor.authorSegundo, Marcela A.
dc.date.accessioned2019-06-27T13:56:00Z
dc.date.available2020-05-02T00:30:29Z
dc.date.issued2019
dc.description.abstractFluoroquinolones are in the order of the day concerning environmental contamination through anthropogenic activities, resulting in increased risk for antibiotic resistance dissemination. In this context, accessible, low-cost analytical methods are required for implementation of comprehensive surveillance and screening schemes. In this work, we propose a down-scaled disk-based solid-phase extraction system from which the eluate can be first screened by miniaturized fluorimetric reading, followed by individual determination of target fluoroquinolones (ciprofloxacin, norfloxacin, and enrofloxacin) by liquid chromatography combined to tandem mass spectrometry. The fluorimetric measurement is based on the intrinsic fluorescence of fluoroquinolones. Disk-based retention was performed after sample acidification (pH 4.0) by mixed-mode cation exchange using polystyrene divinylbenzene sulphonated sorbent. Sample loading was precisely controlled in a dedicated flow system operating at 4.0 mL min−1. Different eluent compositions were tested, with elution performed by 1.00 mL of methanol-ammonium hydroxide (98:2, v/v), with subsequent reading of eluate in both detectors. Quantification was attained for 2–25 µg L−1 range, with LOD values at 1 µg L−1. The proposed approach was successfully applied to estuarine waters from the Douro River, with comparable results to a conventional SPE-LC-MS/MS procedure.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1080/03067319.2019.1588969pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14109
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relationPOCI-01-0145-FEDER-031756pt_PT
dc.relationPOCI-01-0145-FEDER-007265pt_PT
dc.relationPT2020 UID/QUI/50006/2013pt_PT
dc.relationAUTOMATION OF BIOCHEMICAL ASSAYS USING LAB-ON-VALVE AND BEAD INJECTION TECHNIQUES
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.1080/03067319.2019.1588969pt_PT
dc.subjectDisk-based solid-phase extractionpt_PT
dc.subjectFluoroquinolonespt_PT
dc.subjectEnvironmental waterspt_PT
dc.subjectMicroplate fluorimetrypt_PT
dc.subjectTandem mass spectrometrypt_PT
dc.titleScreening of fluoroquinolones in environmental waters using disk-based solid-phase extraction combined to microplate fluorimetric determination and LC-MS/MSpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAUTOMATION OF BIOCHEMICAL ASSAYS USING LAB-ON-VALVE AND BEAD INJECTION TECHNIQUES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCTA-AMB%2F31756%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F89668%2F2012/PT
oaire.citation.endPage269pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage258pt_PT
oaire.citation.titleInternational Journal of Environmental Analytical Chemistrypt_PT
oaire.citation.volume99pt_PT
oaire.fundingStream9471 - RIDTI
person.familyNameBarreiros
person.givenNameLuisa
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id6508205485
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isProjectOfPublication6d890b85-4279-4280-9b7f-2feca7cf3f42
relation.isProjectOfPublicationb191ce5e-496d-4111-b415-7db84a094710
relation.isProjectOfPublication.latestForDiscovery6d890b85-4279-4280-9b7f-2feca7cf3f42

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART_LuísaBarreiros_2019.pdf
Size:
2.17 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESS - FAR - Artigos