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Electrochemical genoassays on gold-coated magnetic nanoparticles to quantify genetically modified organisms (GMOs) in food and feed as GMO percentage

2018-03Journal article Open access
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dc.contributor.authorPlácido, Alexandra
dc.contributor.authorPereira, Clara
dc.contributor.authorGuedes, Alexandra
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorMiranda-Castro, Rebeca
dc.contributor.authorSantos-Álvarez, Noemí de-los-
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2020-01-23T16:39:40Z
dc.date.available2020-01-23T16:39:40Z
dc.date.issued2018-03
dc.description.abstractThe integration of nanomaterials in the field of (bio)sensors has allowed developing strategies with improved analytical performance. In this work, ultrasmall core-shell Fe3O4@Au magnetic nanoparticles (MNPs) were used as the platform for the immobilization of event-specific Roundup Ready (RR) soybean and taxon-specific DNA sequences. Firstly, monodisperse Fe3O4 MNPs were synthesized by thermal decomposition and subsequently coated with a gold shell through reduction of Au(III) precursor on the surface of the MNPs in the presence of an organic capping agent. This nanosupport exhibited high colloidal stability, average particle size of 10.2 ± 1.3 nm, and spherical shape. The covalent immobilization of ssDNA probe onto the Au shell of the Fe3O4@Au MNPs was achieved through a self-assembled monolayer (SAM) created from mixtures of alkane thiols (6-mercapto-1-hexanol and mercaptohexanoic acid). The influence of the thiols ratio on the electrochemical performance of the resulting electrochemical genoassays was studied, and remarkably, the best analytical performance was achieved for a pure mercaptohexanoic acid SAM. Two quantification assays were designed; one targeting an RR sequence and a second targeting a reference soybean gene, both with a sandwich format for hybridization, signaling probes labelled with fluorescein isothiocyanate (FITC), enzymatic amplification and chronoamperometric detection at screen-printed carbon electrodes (SPCE). The magnetogenoassays exhibited linear ranges from 0.1 to 10.0 nM and from 0.1 to 5.0 nM with similar detection limits of 0.02 nM and 0.05 nM for the event-specific (RR) and the taxon-specific (lectin) targets, respectively. The usefulness of the approach was demonstrated by its application to detect genetically modified organisms (GMOs) in feed and food.pt_PT
dc.description.sponsorshipThis work received financial support from the European Union (FEDER funds through COMPETE), National Funds (FCT, Fundação para a Ciência e a Tecnologia) through project UID/QUI/50006/2013 and Regional Funds (Principado de Asturias government through Project FC15-GRUPIN14-025), and cofinanced by FEDER funds. A.P. and M.F.B. are grateful to FCT grants SFRH/BD/97995/2013 and SFRH/BPD/78845/2011, financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. C.P. thanks FCT for the FCT Investigator contract IF/01080/2015.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bios.2018.03.042pt_PT
dc.identifier.issn0956-5663
dc.identifier.urihttp://hdl.handle.net/10400.22/15361
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0956566318302161?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBiosensing Techniquespt_PT
dc.subjectDNA, Plantpt_PT
dc.subjectElectrochemical Techniquespt_PT
dc.subjectGoldpt_PT
dc.subjectMagnetite Nanoparticlespt_PT
dc.subjectPlants, Genetically Modifiedpt_PT
dc.subjectSoybeanspt_PT
dc.subjectNucleic Acid Hybridizationpt_PT
dc.subjectCore-shell Fe3O4@Au magnetic nanoparticlespt_PT
dc.subjectGTS 40-3-2pt_PT
dc.subjectElectrochemical genoassaypt_PT
dc.subjectGenetically modified soybeanpt_PT
dc.subjectGMO quantificationpt_PT
dc.titleElectrochemical genoassays on gold-coated magnetic nanoparticles to quantify genetically modified organisms (GMOs) in food and feed as GMO percentagept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97995%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F78845%2F2011/PT
oaire.citation.endPage154pt_PT
oaire.citation.startPage147pt_PT
oaire.citation.titleBiosensors and Bioelectronicspt_PT
oaire.citation.volume110pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
person.familyNamePlácido
person.familyNamede Sá Barroso
person.familyNameDelerue-Matos
person.givenNameAlexandra
person.givenNameMaria de Fátima
person.givenNameCristina
person.identifier1621053
person.identifier.ciencia-id1B1A-461B-E8DD
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0003-2706-7777
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridL-3085-2014
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id55232677900
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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