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Resumo(s)
Part of the optical clearing study in biological tissues concerns the determination of the diffusion characteristics
of water and optical clearing agents in the subject tissue. Such information is sufficient to characterize the time dependence of the optical clearing mechanisms—tissue dehydration and refractive index (RI) matching. We have used a simple method based on collimated optical transmittance measurements made from muscle
samples under treatment with aqueous solutions containing different concentrations of ethylene glycol (EG), to
determine the diffusion time values of water and EG in skeletal muscle. By representing the estimated mean
diffusion time values from each treatment as a function of agent concentration in solution, we could identify the
real diffusion times for water and agent. These values allowed for the calculation of the correspondent diffusion
coefficients for those fluids. With these results, we have demonstrated that the dehydration mechanism is the
one that dominates optical clearing in the first minute of treatment, while the RI matching takes over the optical
clearing operations after that and remains for a longer time of treatment up to about 10 min, as we could see for EG and thin tissue samples of 0.5 mm.
Descrição
Palavras-chave
Optical clearing Agent diffusion Diffusion coefficient Collimated transmittance Tissue dehydration Refractive index matching
Contexto Educativo
Citação
Editora
Society of Photo-optical Instrumentation Engineers