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- Detection of CA19-9 using fluorescent polymer imprinted membranesPublication . Xu, Ana; Piloto, Ana Margarida L.(Introduction) The Carbohydrate antigen19-9 (CA19-9) is a biomarker widely used for the diagnosis and monitoring of various types of cancer, especially pancreatic cancer (PC). Molecularly Imprinted Polymers (MIPs) are synthetic materials with recognition sites molecularly designed to bind selectively to a target analyte, providing high specificity and sensitivity. In this work, the use of solid supports such as membranes, was used to imprint the MIPs, allowing the creation of robust and easy-to-manipulate biomimetic sensors for the precise and efficient detection of biomarkers, specifically CA19-9.
- Cellulose‑based hydrogel on quantum dots with molecularly imprinted polymers for the detection of CA19‑9 protein cancer biomarkerPublication . Piloto, Ana Margarida L.; Ribeiro, David S.M.; Rodrigues, S. Sofia M.; Santos, João L.M.; Sampaio, Paula; Sales, Maria Goreti FerreiraMolecularly imprinted polymers (MIPs), were successfully assembled around quantum dots (QDs), for the detection of the carbohydrate antigen CA19-9, a biomarker associated with pancreatic cancer (PC). The imprinted materials MIP@QDs were incorporated within cellulose hydrogels and designated as imprinted hydrogels (iHGs). The concept is to use MIPs which function as the biorecognition elements, conjugated to QDs as the sensing system. The fluorescence quenching of the iHGs occurred with increasing concentrations of CA19-9, showing linearity in the range 2.76 × 10−2 – 5.23 × 102 U/ml, in a 1000-fold diluted human serum. The iHGs show a linear response below the cutoff values for pancreatic cancer diagnosis (< 23 U/ml), a limit of detection of 1.58 × 10−3 U/ml and an imprinting factor (IF) of 1.76. Moreover, they exhibit stability and selectivity towards CA19-9, when compared with the non-imprinted controls, here designated as non-imprinted hydrogels (non-iHGs). In conclusion, this work demonstrates that the conjugation of MIPs to QDs increases the sensitivity and specificity of the developed sensing system for optical detection methods within clinical significance ranges. This fact shows potential for the iHGs to be applied as a sensitive, low-cost format for point-of-care tests (PoCTs).
- Paper-based approach for cancer biomarker MMP7 detection using quantum dots and molecularly imprinted polymersPublication . Oliveira, Pedro; Piloto, Ana Margarida L.(Introduction) Preliminary investigation using lab-on-paper Molecular Imprinted Polymers (MIPs) paired with Quantum Dots (QDs) to detect Matrix Metalloproteinase-7 (MMP7) using image processing to quantify fluorescence and area with a CMOS sensor reflex camera. MMP7 is critical in pancreatic tumor formation, which has the lowest survival rate among malignant malignancies. High levels of the MMP7 in blood have been associated to a poor prognosis and limited survival in patients. CdTe Quantum Dots (CdTe@MPA QDs) are luminescent nanoparticles with narrow emission spectra, enabling for exceptional sensitive detection of biomarkers.Combination of MIPs with CdTe MPA-capped provide selectivitive for biomiarker.