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- The history and applications of phage therapy in Pseudomonas aeruginosaPublication . Silva, Carina; Sá, Sara; Guedes, Carla; Oliveira, Carla; Lima, Cláudio; Oliveira, Marco; Mendes, João; Novais, Gonçalo; Baylina, Pilar; Fernandes, RúbenThe Pseudomonas aeruginosa is one of the bacteria that cause serious infections due to resistance to many antibiotics can be fatal in severe cases. Antimicrobial resistance is a global public health concern. To solve this problem, interest in phage therapy has revived; some studies are being developed to try to prove the effectiveness of this therapy. Thus, in this opinion article, several historical aspects are addressed as well some applications of phage therapy against P. aeruginosa.
- In situ crosslinked electrospun gelatin nanofibers for skin regenerationPublication . Dias, J.R.; Baptista-Silva, S.; Oliveira, Carla; Sousa, A.; Oliveira, A.L.; Bártolo, P.J.; Granja, P.L.Due to its intrinsic similarity to the extracellular matrix, gelatin electrospun nanofibrous meshes are promising scaffold structures for wound dressings and tissue engineering applications. However, gelatin is water soluble and presents poor mechanical properties, which generally constitute relevant limitations to its applicability. In this work, gelatin was in situ crosslinked with 1,4-butanediol diglycidyl ether (BDDGE) at different concentrations (2, 4 and 6 wt%) and incubation time-points (24, 48 and 72 h) at 37 °C. The physico-chemical and biological properties of BDDGE-crosslinked electrospun gelatin meshes were investigated. Results show that by changing the BDDGE concentration it is possible to produce nanofibers crosslinked in situ with well-defined morphology and modulate fiber size and mechanical properties. Crosslinked gelatin meshes show no toxicity towards fibroblasts, stimulating their adhesion, proliferation and synthesis of new extracellular matrix, thereby indicating the potential of this strategy for skin tissue engineering.