ESS - TBIO/Rise Health - Posters apresentados em eventos científicos
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- Antimicrobial properties of PLA scaffolds functionalized via LbL method with Manuka and multifloral honeyPublication . Alves, Inês; Ferraz, Ricardo; Prudêncio, Cristina; Rufo, João; Prudêncio, Cristina; Cavaleiro Rufo, JoãoTissue engineering and chronic wound management face obstacles due to the global rise of resistances. Natural bioactive agents, such as Manuka honey, offer a unique therapeutic profile by combining antimicrobial activity with the ability to modulate the biochemical wound environment and promote cellular regeneration. However, the seamless integration of these biological compounds into synthetic matrices, such as polylactic acid (PLA), remains a challenge. This study aims to develop PLA scaffolds functionalised with honey using the Layer-by-Layer (LbL) technique and evaluate their inhibitory capacity against microbial growth. Two honey types are com-pared, MGO 263+/UMF10+ Manuka honey and a Portuguese multifloral honey. Concentrations of 15%, 20%, 25%, 30% and 35% will be tested by diluting the honey in 3M sodium acetate buffer and adjusting pH to 5.5. The method has currently been successfully optimized for the 15% concentration. The LbL process consist-ed of three immersion cycles, alternating with coating in the honey solutions, washing in acetate buffer and short periods of drying. As a negative control, PLA scaffolds were subjected only to the washing steps. Biological activity was assessed against Escherichia coliand Staphylococcus aureus. In the disc diffusion test, the plates were inoculated uniformly and the scaffolds arranged in quadrants, including positive controls of ciprofloxacin for E. coli andpenicillin G for S. aureus. After incubation, inhibition was quantified by two per-pendicular measurements of the halo. Quantification of Colony-Forming Units (CFU) was performed after recovery of the microorganisms from the scaffolds by vortexing and centrifugation in saline solution. The study is currently in the optimization phase. Sterilization has been successfully achieved by im-mersing the scaffolds in 70% ethanol for 2 hours with gentle stirring, followed by vacuum drying in a Petry dish for 24 hours. Microscopic observation of the scaf-folds showed a clear coating layer in functionalized samples. This research seeks to validate LbL functionalisation as a simple strategy for developing bioactive scaffolds. The present work establishes a foundation for subsequent phases involving various honey concentrations, with the goal of demonstrating a synergistic effect between the PLA scaffold and nat-ural antimicrobial agents.
- Creation of a fungal library and screening of antimicrobial and anticancer activityPublication . Ferreira, Diogo; Hermida, Lara Areal; Rocha, Ana Catarina; Baylina, Pilar; Sieiro, Carmen; Fernandes, Rúben; BAYLINA MACHADO, PILARAccording to the World Health Organization, cancer and infectious diseases are two of the most problematic diseases nowadays. Cancer kills 10 million people every year and the emergence of resistance to antitumoral drugs is an important medical challenge. At the same time, antimicrobial resistance (AMR) is also a serious threat to human and environmental health. Besides mortality, AMR burdens healthcare services and dampens medical procedures such as surgeries, cancer treatments and other invasive procedures. The development of new drug therapies to fight drug resistance is essential to contest the rising of resistant bacteria and reduction of the effectiveness of antitumoral drugs. Microorganisms have been a major source for natural compounds throughout the years. Fungi, renowned for their ability to produce an array of broad and diverse secondary metabolites, offer a rich resource for drug discovery. We built a collection of fungal species, isolated from chestnuts, sunflower seeds, and chestnut flour, and explored their extracts for potential antimicrobial and anticancer activity. Fungi cultures for secondary metabolite biosynthesis were done in submerged fermentation in Malt Extract broth for 15 days at 26 °C. Liquid-liquid extraction techniques, with ethyl acetate as a solvent, were applied to obtain crude secondary metabolite extracts. Clinical resistant bacteria, yeasts, and prostate cell lines (human prostate epithelial cells – HpepiC; human caucasian prostate adenocarcinoma cells - PC3) were exposed to fungal extracts at a single concentration of 100 µg/mL. Our results so far show several extracts with antimicrobial and/or anticancer activity without decreasing cell viability of non-tumoral cells, showing their potential as therapeutic drugs without possible secondary effects. Although, more studies should be done, and pending fungal identification will allow us to select which extracts will be further investigated to find if the displayed bioactivity could be happening due to unknown natural compounds