ESS - CM - Posters apresentados em eventos científicos
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Browsing ESS - CM - Posters apresentados em eventos científicos by Subject "Acetylcholinesterase"
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- Evaluation of the potential of marine cyanobacteria towards Alzheimer’s diseasePublication . Moreira, Ana Rita; Silva, Ana Margarida; Vieira, Mónica; Martins, RosárioAlzheimer’s disease (AD) is one of the most prevalent neurodegenerative diseases (NDs) and the major cause of dementia. Despite the constant efforts to achieve cure, no treatment is currently effective and new approaches are urgently needed. Marine biotechnology has been at the frontline for the bioprospection of natural sources to treat ND. Several marine compounds have already provided evidence for anti-AD drugs by acting in mechanisms such as the inhibition of acetylcholinesterase (AChE). Among marine organisms, cyanobacteria have been recognized as a major source of bioactive compounds and the potential towards AD has been highlighted, namely AChE inhibitors. This project is included in a screening program that aims to evaluate the potential of cyanobacteria from the Culture Collection of CIIMAR -LEGE-CC against AD. In this work, 80 fractions of marine cyanobacteria were screened for the potential to inhibit AChE since this is a proven enzyme target to ameliorate the loss of cholinergic signal and to induce cytotoxicity in the neuroblastoma cell line SHY5Y, in the normal fibroblasts cell line 3T3L1, and in the endothelial cells from central nervous system capillaries hCMEC/D3. Results considering the enzymatic assays and cytotoxic assays revealed that the fractions are not toxic or with low toxicity. The strains Phormidium sp; an unidentified filamentous Synechococcales and Nodosilinea nodulosa revealed moderate toxicity towards AChE.
- Neuroprotective effects of cyanobacterial extractsPublication . Rodrigues, Flávia; Grosso, Clara; Ferreira, Leonor; Vasconcelos, Vítor; Reis, Mariana; Martins, Rosário; Martins, RosarioNeurodegenerative diseases (ND), namely Alzheimer’s disease (AD) affects millions of people worldwide. Despite their high incidence there is still no effective medical therapy. Cyanobacteria have been in the frontline of the search for therapies for ND. Several in silico, in vitro, and in vivo studies have provided support for the neuroprotective potential of cyanobacterial natural products, particularly in AD. In this context, the aim of this work was to screen cyanobacterial strains from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE-CC) for its anti-AD potential. 10 cyanobacterial strains (LEGE CC 06072, 06131, 06155, 06361, 07168, 07175, 07189, 11394, 11439, 16525) were cultivated and their dry biomass was extracted and fractionated by reverse-phase HPLC resulting in 8 fractions per strain. Fractions were evaluated for their ability to inhibit enzymes associated with AD pathogenesis, such as acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), using Ellman’s colorimetric method. In addition, their cytotoxicity was assessed against three cell lines, two of them related to the nervous system. The neuroblastoma cell line SH-SY5Y, usually used as model for neurotoxicity studies and the human cerebral microvascular endothelial cell line hCMEC/D3, representative of the blood-brain barrier. The mouse fibroblast cell line 3T3-L1 was used as a representation of the conjunctive tissue. Cell viability was measured after 24 and 48 hours using the MTT assay. Results: The fractions did not shown cytotoxicity against the cell lines used. The preliminary results regarding enzyme inhibition were not promising, with less than 30% inhibition. Our preliminary suggest that further confirmation tests need to be performed on the AD related enzymes (AChE and BuChE), and the study should be extended to include other cyanobacterial strains.