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Browsing by Issue Date, starting with "2023-09-13"

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  • PML/ RARA variants and their role in Arsenic Trioxide resistance in APL: a scoping review
    Publication . Gonçalves, Patrícia; Santos, Claúdia dos; Sousa, Maria; Mota, Sandra
    Currently, the treatment of Acute Promyelocytic Leukemia (APL) is grounded on therapeutic regimens based on arsenic trioxide (ATO). However, mutations in the PML component of the PML/RARA oncoprotein are believed to be involved in the mechanism of resistance to this agent. We performed a scoping review to understand the role of variant PML/RARA fusion proteins in the mechanism of resistance in APL. Applying the defined criteria, we selected 10 studies, in which patients presented a picture of relapse and/or resistance after the administration of ATO, having been identified at least one PML mutation. We also reported that RARA is the most frequently mutated gene, although we also found mutations in genes related to other processes involved in cell differentiation. Briefly, this is a multifactorial mechanism and PML/RARA variants are important but not an obligatory condition for ATO resistance to occur.
    2023-09-13Book part Open access Show more
  • Adverse reactions mediated by Strontium-89, Samarium-153, Rhenium-186 and Rhenium-188: A Systematic Review of Literature
    Publication . Pinho, Cláudia; Martins, Sara; Jesus, Ângelo; Suárez, Ana Martín
    One of the therapies used for the palliative treatment of pain associated with bone metastases is radiopharmaceutical therapy. Radiopharmaceuticals can cause adverse reactions, and, therefore, the aim is to systematize the results and conclusions of studies on the use of the radiopharmaceuticals 89Sr, 153Sm, 186Re and 188Re in the palliative treatment of pain associated with bone metastases. A systematic literature review was conducted according to PRISMA statement, using the databases MEDLINE and EBSCO. After the selection process, 20 articles were included. The studies showed that the 4 radiopharmaceuticals analysed presented very similar results regarding pain relief after treatment, decrease in analgesic consumption, side effects at the time of administration, hematologic toxicity and disease progression after treatment. Concluding, the use of radiopharmaceuticals for pain palliation seems to be safe and an alternative to existing treatments.
    2023-09-13Book part Open access Show more
  • Optimization of an HPLC-DAD method for Quercetin detection
    Publication . Carvalho, Daniel; Moreira, Fernando; Oliveira, Ana Isabel
    High-performance liquid chromatography (HPLC) is one of the most used liquid chromatographic techniques owing to its versatility that allows an optimal resolution. Quercetin (QUE), a polyphenol, has received increasing attention due to its antioxidant properties. Efforts have been made to overcome the traditional poor oral bioavailability of quercetin, requiring the development of analytical techniques for quercetin detection in different matrices. The main objective of this work was the optimization of a HPLC-Diode Array Detector (DAD) method for quercetin. An optimization process was conducted to guarantee the best conditions and results. Detection and quantification were executed with a reverse phase C-18 column. Different mobile phase (MP) compositions, flow rates, and wavelengths (with DAD) were studied. Better results were achieved with a MP composition of water:acetonitrile:methanol (55:40:5), with 1.5% acetic acid at a 1.3 mL/minute flow rate, with good results regarding chromatogram resolution, and quercetin quantification and detection.
    2023-09-13Book part Open access Show more