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- Tissue optical clearing as a diagnostic tool for tissue pathology differentiationPublication . Oliveira, Luís; Carneiro, Isa; Carvalho, Sónia; Henrique, Rui; Tuchina, Daria; Timoshina, Polina; Bashkatov, Alexey; Genina, Elina; Tuchin, ValeryWith the objective of developing a diagnostic tool, we have used the immersion optical clearing method and studied normal and pathological tissues (cancer, diabetes) under treatment by optical clearing agents (OCAs). In order to quantify pathology status OCA diffusion properties in different tissues were measured. We have demonstrated that free water content in cancerous tissues is higher than in normal.