Browsing by Author "Zengin, Gokhan"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
- A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus LPublication . Cvetanović, Aleksandra; Zeković, Zoran; Švarc-Gajić, Jaroslava; Razić, Slavica; Damjanović, Ana; Zengin, Gokhan; Delerue-Matos, Cristina; Moreira, Manuela M.BACKGROUND To obtain bioactive fractions from plant material subcritical water is notably advantageous in comparison to any other technique. Excellent solvating properties and selectivity combined with finely tuned reactivity of subcritical water enable exploitation of the potential of plants. Herein subcritical water extraction was used for recovery of bioactive compounds from leaves, roots and fruits of Sambucus ebulus L. Extracts obtained were characterized in terms of biological and chemical fingerprints. RESULTS AND DISCUSSION Results obtained by using several antioxidant assays that focused on different mechanisms showed that subcritical water extracts of Sambucus ebulus were powerful antioxidants. Enzyme inhibitory effects were tested against α‐amylase, α‐glucosidase and tyrosinase, and the results showed the anti‐diabetic potential of the extracts as well as its possible use with skin disorders. Antiproliferative properties were detected on three cell lines (A‐549; LS‐174 T; HeLa) and showed prominent cytotoxicity against all tumor cell lines. Inhibitory concentrations obtained were in the range 0.58–8.10 μg mL‐1. Generally, the SCW extracts from leaves exhibited stronger biological activities with higher levels of phenolic compounds compared with the roots and fruits of Sambucus ebulus. Gallic acid, catechin and caffeic acid were identified as major components in these extracts and these components seem to relate with observed biological activity. CONCLUSION The results obtained suggest that Sambucus ebulus has great potential for preparing new phyto‐pharmaceuticals and functional food ingredients. © 2017 Society of Chemical Industry
- The influence of the extraction temperature on polyphenolic profiles and bioactivity of chamomile (Matricaria chamomilla L.) subcritical water extractsPublication . Cvetanović, Aleksandra; Švarc-Gajić, Jaroslava; Zeković, Zoran; Jerković, Jelena; Zengin, Gokhan; Gašić, Uroš; Tešić, Živoslav; Mašković, Pavle; Soares, Cristina; Barroso, M. Fátima; Delerue-Matos, Cristina; Đurović, SašaThe main goal of this research was to determine the relationship among chemical structure, bioactivity and temperature of chamomile during subcritical water extraction in isobaric conditions (45 bar) at seven different temperatures (65-210 °C). The influence of temperature on phenolic profiles was defined by UHPLC-HESI-MS/MS. The overall results indicate that the presence of conjugated double bonds, side chains, glucose moiety or ether moiety in molecules influence the efficiency of polyphenols' extraction in subcritical water. In terms of antioxidant activity, the extracts were the most active towards ABTS radicals (IC50 = 7.3-16.8 µg/mL), whereby temperature of 150 °C was optimal. On the other hand, the extracts obtained at 115 °C showed highest cytotoxicity. Inhibition of α-amylase and α-glucosidase was the highest at 65 and 85 °C, i.e. 0.51 and 4.13 mmolAE/g, respectively. Activity against tyrosinase was the highest at 210 °C (17.92 mgKAE/g). The data showed that different non-phenolic compounds may also participate in bio-activities of the extracts.