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Percorrer por autor "Pinto, A.S."

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  • E-Commerce Website Accessibility Assessment Portugal Case Overview
    Publication . Afonso, A.P.; Pinto, A.S.; Braga, I.; Oliveira, R.; Braga, Inês; Afonso, Ana Paula; Sousa Pinto, Agostinho
    The World Wide Web, boosted the development and exponential growth, worldwide, of numerous online businesses. Most of organizations in the developed countries are present, in some way, online. It is based on this assumption, that digital accessibility plays a fundamental role in democratizing the use of online technologies for all citizens, including the most physically disabled: with motor or perceptive difficulties. A website could be considered accessible if it is also functionally usable, which means, easiness to interpret the content as well as navigating through the web pages, offering the customer a satisfying and inclusive experience. In this context, we propose to develop an accessibility assessment tool, ShoppingForAll, targeting users with a low degree of disability, in accordance with the Web Content Accessibility Guidelines (WCAG 2.1) for level A of compliance. This new tool will be applied to a restricted set of online stores, national or with an infrastructure in Portugal. The criteria used to select the websites under the study were native language and the position in the sales ranking. After analysing the results obtained, it was possible to establish a set of recommendations aimed at improving the accessibility of e-commerce websites. Our contribute aimed to promote and increase, albeit modestly, the accessibility on the Web. This tool can be used by experts in information technologies, by developers, and possibly e-commerce website managers.
    2022Livro Acesso aberto Ver mais
  • Protective effect of C. sativa leaf extract against UV mediated-DNA damage in a human keratinocyte cell line
    Publication . Almeida, Isabel F.; Pinto, A.S.; Monteiro, C.; Monteiro, H.; Belo, L.; Fernandes, J.; Bento, A.R.; Duarte, T.L.; Garrido, J.; Bahia, Maria Fernanda; Lobo, J.M. Sousa; Costa, P.C.
    Toxic effects of ultraviolet (UV) radiation on skin include protein and lipid oxidation, and DNA damage. The latter is known to play a major role in photocarcinogenesis and photoaging. Many plant extracts and natural compounds are emerging as photoprotective agents. Castanea sativa leaf extract is able to scavenge several reactive species that have been associated to UV-induced oxidative stress. The aim of this work was to analyze the protective effect of C. sativa extract (ECS) at different concentrations (0.001, 0.01, 0.05 and 0.1 μg/mL) against the UV mediated-DNA damage in a human keratinocyte cell line (HaCaT). For this purpose, the cytokinesis-block micronucleus assay was used. Elucidation of the protective mechanism was undertaken regarding UV absorption, influence on 1O2 mediated effects or NRF2 activation. ECS presented a concentration-dependent protective effect against UV-mediated DNA damage in HaCaT cells. The maximum protection afforded (66.4%) was achieved with the concentration of 0.1 μg/mL. This effect was found to be related to a direct antioxidant effect (involving 1O2) rather than activation of the endogenous antioxidant response coordinated by NRF2. Electrochemical studies showed that the good antioxidant capacity of the ECS can be ascribed to the presence of a pool of different phenolic antioxidants. No genotoxic or phototoxic effects were observed after incubation of HaCaT cells with ECS (up to 0.1 μg/mL). Taken together these results reinforce the putative application of this plant extract in the prevention/minimization of UV deleterious effects on skin.
    2015Artigo científico Acesso restrito Ver mais