Browsing by Author "Pereira, C."
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- Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approachPublication . Rodrigues, F.; Pereira, C.; Pimentel, F. B.; Alves, R. C.; Ferreira, M.; Sarmento, B.; Amaral, Maria Helena; Beatriz, M.; Oliveira, P. P.Recent changes in regulatory requirements and social views on animal testing have incremented the development of reliable alternative tests for predicting skin and ocular irritation potential of products based on new raw materials. In this regard, botanical ingredients used in cosmetic products are among those materials, and should be carefully reviewed concerning the potential presence of irritant constituents. In particular, cosmetic products used on the face, in vicinity of the eyes or that may come in contact with mucous membranes, should avoid botanical ingredients that contain, or are suspected to contain, such ingredients. In this study, we aimed to evaluate the effect of a new cosmetic ingredient, namely, coffee silverskin (CS), with an in vitro skin and ocular irritation assay using reconstructed human epidermis, EpiSkin™, and human corneal epithelial model, SkinEthics™ HCE, and an in vivo assay. Three different extracts of CS were evaluated. The histology of the models after extracts applications was analysed. The in vitro results demonstrated that extracts were not classified as irritant and the histological analyses proved that extracts did not affect both models structure. The content of caffeine, 5-hydroxymethyl furfural and chlorogenic acid was quantified after the epidermal assay. The in vivo test carried out with the most promising extract (hydroalcoholic) showed that, with respect to irritant effects, these extracts can be regarded as safe for topical application.
- A factorial design for optimization of the analytical variables on the development of a genoassay for the transgenic soybean detectionPublication . Ramos-Jesus, J.; Barroso, M. Fátima; M. S. Silva, Luís; Pereira, C.; Freire, C.; de-los- Santos-Álvarez, N.; Leite, José Roberto S.A.; Delerue-Matos, CristinaAt the laboratory, analytical method optimizations are performed to achieve the maximum sensitivity and selectivity. Routinely, this procedure is carried out by optimizing one-factor-at-a-time approach until there is no further improvement, where each experimental parameter is optimized separately and independently of the other factors.
- A hiperglicemia e o secretoma de adipócitos alteram o padrão de crescimento de micobactérias in vitroPublication . Fernandes, Rúben; Bronze, M.; Pereira, C.; Coelho, Pedro; Prudêncio, Cristina
- Institutional factors governing the deployment of remote experiments: lessons from the rexnet projectPublication . Hine, Nick; Alves, Gustavo R.; Erbe, Heinz-H; Muller, Dieter; Alves, J. B. M.; Pereira, C.; Ferreira, José M.; Sucar, Enrique; Herrera, Oriel; Chiang, Luciano; Garcia-Zúbia, JavierRemote labs offer many unique advantages to students as they provide opportunities to access experiments and learning scenarios that would be otherwise unavailable. At the same time, however, these opportunities introduce real challenges to the institutions hosting the remote labs. This paper draws on the experiences of the REXNET project consortium to expose a number of these issues as a means of furthering the debate on the value of remote labs and the best practices in deploying them. The paper presents a brief outline of the various types of remote lab scenarios that might be deployed. It then describes the key human and technological actors that have an interest in or are intrinsic to a remote lab instance, with a description of the role of each actor and their interest. Some relationships between these various actors are then discussed with some factors that might influence those relationships. Finally some general issues are briefly described.
- Powered smart textile-based exoskeleton for human support movementPublication . Ferreira, C.; Fertuzinhos, A.; Silva, R.; Ramalho, M.; Vale, B.; Silva, J.; Costa, L.; Oliveira, C.; Ramôa, A.; Aguiar, F.; Pilastri, A.; Matta, A.; Dias, P.; Sampaio, R.; Machado, D.; Costa, M.; Roças, A.; Madureira, P.; Moreira, Juliana; Pereira, J. R.; Pereira, C.; Pereira, F. B.Industrial environments require people’s physical efforts related to excessive force, poor posture, and repetitive tasks that increase the risk of developing musculoskeletal diseases. Due to the high prevalence of postural complaints and consequent global health burdens, a significant demand has been conducted to build and implement powered lower and upper limb devices for human enhancement, considering the working layout/position, anthropometric and ergonomic data to generate valuable information for posture correction guidance.