Browsing by Author "Neuparth, T."
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- Single Low-Dose Ionizing Radiation Induces Genotoxicity in Adult Zebrafish and its Non-Irradiated ProgenyPublication . Lemos, Joana; Neuparth, T.; Trigo, M.; Costa, Pedro; Vieira, Domingos; Cunha, L.; Ponte, F.; Costa, P. S.; Metello, Luís; Carvalho, A. P.This study investigated to what extent a single exposure to low doses of ionizing radiation can induce genotoxic damage in irradiated adult zebrafish (Danio rerio) and its non-irradiated F1 progeny. Four groups of adult zebrafish were irradiated with a single dose of X-rays at 0 (control), 100, 500 and 1000 mGy, respectively, and couples of each group were allowed to reproduce following irradiation. Blood of parental fish and whole-body offspring were analysed by the comet assay for detection of DNA damage. The level of DNA damage in irradiated parental fish increased in a radiation dose-dependent manner at day 1 post-irradiation, but returned to the control level thereafter. The level of DNA damage in the progeny was directly correlated with the parental irradiation dose. Results highlight the genotoxic risk of a single exposure to low-dose ionizing radiation in irradiated individuals and also in its non-irradiated progeny.
- Study of radiobiological effects of low doses of ionizing radiation using comet assay on zebrafish modelPublication . Lemos, Joana; Costa, Pedro; Cunha, Lídia; Carvalho, A.; Neuparth, T.; Vasconcelos, Vítor; Genésio, P.; Ponte, F.; Costa, P.; Crespo, P.; Metelo, Luís F.Present paper relates with the analysis of radiobiological effects, in a context of ionizing radiation low doses, using an advanced biological model, the zebrafish - Danio rerio - and comet assay based analytical methodology. The use of zebrafish has grown considerably in recent years, assuming more and more is role as a very promising model in biomedical research, both because of the high level of homology shared with the human genome, and an assumedly easy - and reasonably priced - practical side. To obtain the data here mentioned, it has been used a population of circa 300 animals, divided in four groups of 75 each, all having been externally irradiated with three distinct dose protocols: 100, 500 and 1.000 mGy. The postures of the irradiated fish were collected and groups of adult animals were killed in three different moments: 1, 4 and 7 days after the irradiation. DNA damage in adults and in larvae were studied using comet assay. The results related to damages were processed and presented in terms of percentual of DNA in tail, allowing an approximation to the relative number of DNA breaks induced by the radiation exposure.