Browsing by Author "Gojon, Frantz"
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- Soft Skills aplicadas: Desafios e reflexões sobre a organização de um evento científico onlinePublication . Sousa, Zita Castorina Ribeiro de; Lopes, Andreia; Nogueira, Cláudia; Gojon, Frantz; Ferreira, Inês; Gomes, InêsIntegrada no último ano da Licenciatura em Biotecnologia Medicinal da ESS|P.Porto, a Unidade Curricular “Competências-Transversais” visa potenciar o desenvolvimento de competências pessoais, sociais e de empreendedorismo dos estudantes, bem como a aquisição de competências de empregabilidade. Especificando, promove aptidões como a criatividade e geração de ideias, trabalho de equipa e cooperação, análise do mercado de trabalho e áreas de investigação, gestão de projetos, de tempo e de stress, ferramentas de comunicação profissional, networking, etc. Estas competências são estimuladas através da organização - em autogestão - de um evento científico pelo grupo de estudantes, supervisionado pela docente. O grupo decide e aplica: da ideia ao plano, ao processo-de-ação, ao resultado/evento, e à reflexão sobre o processo-e-resultado. Em suma, é o grupo que assume o planeamento, desenvolvimento e avaliação do evento. As dificuldades e desafios impostos pela pandemia COVID-19 implicaram mudanças neste trabalho, surgindo a necessidade de se adotar uma abordagem remota para um evento, que ocorre habitualmente em formato presencial.
- The synergic effect of antibiotics is dependent of oxidative stress genes in Pseudomonas aeruginosaPublication . Fernandes, Rúben; Coelho, Pedro Barata; Guedes, Carla; Gojon, Frantz; Oliveira, Marco; Veiga, Marlene; Sá, Sara; Silva, Carina; Baylina, PilarPseudomonas aeruginosa is a Gram-negative opportunistic pathogen commonly found in Cystic fibrosis, infected wound of the diabetic foot among others. Clinical management of such infection depends deeply on the antibiotic therapy. Antibiotic response is dependent, among several other factors, to the response to host stress conditions, such as low-grade inflammation, metabolic conditions and oxidative stress and to social bacteria response such as quorum sensing and biofilm formation. P. aeruginosa, in particular strain PAO1 is also a biological model for studying bacterial biofilm formation. The present study aims to understand the antibiotic synergic response (ampicillin, ceftazidime, ciprofloxacin) in biofilm formation / degradation of 10 PAO1 oxidative gene mutants. Viability was measured by means of Erythrosin B and biofilm formation was measured by Crystal Violet assay. PAO1 GRLX, SEPHS 1, Rub A1, where the strains with a most pronounced biofilm formation and combination of ceftazidme::ciprofloxacin were most efficient in this biological model. The results are interesting, and although they are encouraging, they should be taken with caution.
- Which role plays 2-Hydroxybutyric acid on Insulin resistance?Publication . Sousa, André P.; Cunha, Diogo M.; Franco, Carolina; Teixeira, Catarina; Gojon, Frantz; Baylina, Pilar; Fernandes, RúbenType 2 Diabetes Mellitus (T2D) is defined as a chronic condition caused by beta cell loss and/or dysfunction and insulin resistance (IR). The discovering of novel biomarkers capable of identifying T2D and other metabolic disorders associated with IR in a timely and accurate way is critical. In this review, 2-hydroxybutyric acid (2HB) is presented as that upheaval biomarker with an unexplored potential ahead. Due to the activation of other metabolic pathways during IR, 2HB is synthesized as a coproduct of protein metabolism, being the progression of IR intrinsically related to the increasing of 2HB levels. Hence, the focus of this review will be on the 2HB metabolite and its involvement in glucose homeostasis. A literature review was conducted, which comprised an examination of publications from different databases that had been published over the previous ten years. A total of 19 articles fulfilled the intended set of criteria. The use of 2HB as an early indicator of IR was separated into subjects based on the number of analytes examined simultaneously. In terms of the association between 2HB and IR, it has been established that increasing 2HB levels can predict the development of IR. Thus, 2HB has demonstrated considerable promise as a clinical monitoring molecule, not only as an IR biomarker, but also for disease follow-up throughout IR treatment.