Browsing by Author "Caires, Hugo"
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- 3D vs 2D Cell Cultures in the Evaluation of Radiobiological Effects of Exposition to Low Doses - Medical Imaging Levels - of Ionizing RadiationPublication . Costa, Pedro; Caires, Hugo; Lemos, Joana; Cunha, Lídia; Bravo, Joana; Bravo, Isabel; Silva, Regina; Summavielle, Teresa; Metello, Luís F.Pretending to develop advanced biological models to study biological effects of low doses of ionizing radiation and following the actual policies on Animal Sciences, based on 3 R’s Rule (to Reduce, Refine and Replace) – that limits as much as possible the application of animal models – scientific research using cellular models is constantly increasing. Nevertheless, the intrinsic limitations of actual cellular models quite often had been recognized on a significant number of papers pointing a significant number of non-concordances between results obtained using in vitro and in vivo studies. Actually, an increasing number of authors admit that three-dimensional cell culture (and spheroid cell culture in particular) could represent an interesting solution and a step further on use of cellular models. The work here to be presented reflects the first phase on the use of this methodology on the study, evaluation and quantification of cellular effects of low doses – starting on medical imaging level - of exposition to ionizing radiation.
- Zebrafish and Nuclear Medicine methods and techniques: an each-day improving partnershipPublication . Lemos, Joana; Metello, Luís F.; Caires, Hugo; Costa, Pedro; Martins, Rosario; Bravo, Isabel; Carvalho, António Paulo; Vasconcelos, VítorAims: This work aims the introduction of zebrafish as a very promising and each day more interesting animal model in radiobiology. It will be briefly discussed the most relevant advantages and disadvantages related with the use of zebrafish, mentioning some of the previous studies using this model in Nuclear Medicine and concluding with its potential applications.