Browsing by Author "Azenha, Manuel"
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- Development of a chromatographic method for simultaneous analysis of glutathione formsPublication . Teixeira, Dulce; Coelho, Pedro; Prudêncio, Cristina; Azenha, Manuel; Vieira, MónicaReduced glutathione (GSH)is the most abundant low molecular weight thiol-containing tripeptide (glycine, cysteine, and glutamate) which is synthesized in the cells. GSHplays critical roles in protecting cells from oxidative damage and the toxicity of xenobiotics. Besides, it is also involved in the regulation of intracellular redox homeostasis, which leads to its oxidation into oxidized glutathione (GSSG). Determining the ratio of GSH/GSSG in different biological samples is a major procedure for the evaluation of an individual’s oxidative status and can be a potential biomarker of oxidative stress.
- Development of a new method for the detection and quantification of octopamine and 3-nitrotyrosinePublication . Teixeira, Dulce; Prudêncio, Cristina; Azenha, Manuel; Vieira, MónicaNeurodegenerative diseases affect around 30 million people worldwide, thus new methods that can facilitate research of such diseases are essential. Neurotransmitters, characterised as critical regulators of several neurological disorders, have been the target for the development of such methods. Besides, oxidative/ nitrosative stress has been associated with several neurodegenerative diseases and is thought to play an important role in their pathogenesis. Therefore, the development of bioanalytical methods for quantification of neurotransmitters and oxidative stress-associated molecules seems to be an interesting approach. The aim of this study was to develop a simple, rapid, low-cost and sensitive octopamine (OCT) and 3-nitrotyrosine (3-NT) quantification method for use in biological models.