Browsing by Author "Araújo, Alyne Rodrigues de"
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- Acetylated cashew gum-based nanoparticles for the incorporation of alkaloid epiisopiloturinePublication . Rodrigues, Jessica do Amaral; Araújo, Alyne Rodrigues de; Pitombeira, Nadia Aline; Plácido, Alexandra; Almeida, Miguel Peixoto de; Veras, Leiz Maria Costa; Delerue-Matos, Cristina; Lima, Filipe Camargo Dalmatti Alves; Neto, Augusto Batagin; Paula, Regina Célia Monteiro de; Feitosa, Judith Pessoa Andrade; Eaton, Peter; Leite, José Roberto Souza A.; Silva, Durcilene Alves daThe natural alkaloid epiisopiloturine has recently become the focus of study for various medicinal properties, particularly for its anti-inflammatory and antischistosomal effect. The incorporation of active molecules in natural polymeric matrices has garnered increasing interest during recent decades. A new derivative of cashew gum successfully obtained by gum acetylation has shown great potential as a carrier in controlled drug release systems. In this work, epiisopiloturine was encapsulated in acetylated cashew gum nanoparticles in order to increase solubility and allow slow release, whereas the morphology results were supported by computer simulations. The particles were produced under a variety of conditions, and thoroughly characterized using light scattering and microscopic techniques. The particles were spherical and highly stable in solution, and showed drug incorporation at high levels, up to 55% efficiency. Using a dialysis-based in vitro assay, these particles were shown to release the drug via a Fickian diffusion mechanism, leading to gradual drug release over approximately 6 h. These nanoparticles show potential for the use as drug delivery system, while studies on their potential anti-inflammatory action, as well as toxicity and efficacy assays would need to be performed in the future to confirm their suitability as drug delivery candidates.
- Copper nanoparticles stabilized with cashew gum: Antimicrobial activity and cytotoxicity against 4T1 mouse mammary tumor cell linePublication . Amorim, Adriany; Mafud, Ana Carolina; Nogueira, Silvania; Ramos-Jesus, Joilson; Araújo, Alyne Rodrigues de; Plácido, Alexandra; Neta, Maria Brito; Alves, Michel Muálem Moraes; Carvalho, Fernando Aécio Amorim; Rufino Arcanjo, Daniel Dias; Braun, Sacha; López, Marta Sánchez-Paniagua; López-Ruiz, Beatriz; Delerue-Matos, Cristina; Mascarenhas, Yvonne; Silva, Durcilene; Eaton, Peter; Leite, José Roberto Souza AlmeidaCopper nanoparticles stabilized with cashew (CG-CuNPs) were synthesized by reduction reaction using ascorbic acid and sodium borohydride, using the cashew gum (CG) as a natural polymer stabilizer. Dynamic light scattering, atomic force microscopy, Fourier-transform infrared spectroscopy, UV-Vis spectrophotometry, and x-ray diffraction were used to characterize the nanoparticles (CG-CuNPs), and copper was quantified by electrochemical measurement. The UV-vis spectra of the CG-CuNPs confirmed the formation of nanoparticles by appearance of a surface plasmon band at 580 nm after 24 h of reaction. The Fourier-transform infrared spectrum of CG-CuNPs showed the peak at 1704 cm−1 from cashew gum, confirming the presence of the gum in the nanoparticles. The average size of CG-CuNPs by dynamic light scattering and atomic force microscopy was around 10 nm, indicating small, approximately spherical particles. Antimicrobial assays showed that CG-CuNPs had activity against Staphylococcus aureus ATCC 29213 with a minimal inhibitory concentration of 0.64 mM. The cytotoxicity assay on BALB/c murine macrophages showed lower cytotoxic effects for CG-CuNPs than CuSO4·5H2O. Viability cell assays for CG-CuNPs at (0.250 mM) inhibited by 70% the growth of 4T1 LUC (4T1 mouse mammary tumor cell line) and NIH 3T3 cells (murine fibroblast cells) over a 24-h period. Therefore, CG-CuNPs can be used as an antimicrobial agent with lower cytotoxic effects than the CuSO4·5H2O precursor.