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A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker

2020Journal article Open access
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dc.contributor.authorResende, Sara
dc.contributor.authorFrasco, Manuela F.
dc.contributor.authorSales, Maria Goreti Ferreira
dc.date.accessioned2021-09-28T08:48:45Z
dc.date.available2021-09-28T08:48:45Z
dc.date.issued2020
dc.description.abstractThis work reports a novel label-free sensor combining photonic crystals and molecularly-imprinted polymer materials for targeting a protein in human samples within levels of clinical interest. This concept was applied to detect fibrinopeptide B (FPB) in urine, a biomarker of venous thromboembolism, which is a disease of great concern and demands innovative point-of-care devices for improved diagnosis. The molecularly-imprinted photonic polymer (MIPP) was obtained by tailoring an imprinted polymer on highly ordered silica nanoparticles assembled by vertical deposition. Owing to the hierarchical structure, the resulting MIPP exhibited optical properties that changed upon rebinding of the target analyte, FPB. Thus, the changes in reflectance intensity enabled a rapid and sensitive detection of FPB in human urine. The observed response was linear between 0.2 ng mL−1 and 22 ng mL-1 and showed a limit of detection of 0.13 ng mL-1. These features of the sensing material allow the assessment of urinary FPB at relevant clinical levels. In addition, the sensor was selective for FPB compared to the standard biomarker of venous thromboembolism, D-Dimer. The stability of the material was evidenced by the reusability study, which demonstrated reversibility of the maximum intensity after three cycles of recognition and regeneration. Moreover, the sensor showed good performance for the spiked FPB detection in control human urine. Overall, the application of such label-free sensor offers high selectivity and stability, as well as easy operation. It may constitute an alternative method for non-invasive and real-time detection of various protein biomarkers in point-of-care.pt_PT
dc.description.sponsorshipThe authors gratefully acknowledge the financial support from FEDER (european regional development fund), Fundação para a Ciência e Tecnologia (FCT) and INTERREG (previously missing here), through projects STRIP2SENSE (NORTE-01-0145-FEDER-024358-SAICT-POL/24358/2016) and IBEROS (Instituto de Bioingeniería en Red para el Envejecimiento Saludable, INTERREG POCTEP/0245_IBEROS_1_E). Sara Resende thanks FCT for the PhD grant ref. SFRH/BD/139634/2018.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationResende S, Frasco MF, Sales MGF, A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker, Sensors and Actuators: B. Chemical (2020), doi:https://doi.org/10.1016/j.snb.2020.127947pt_PT
dc.identifier.doi10.1016/j.snb.2020.127947pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/18590
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-01-0145-FEDER-024358-SAICT-POL/24358/2016pt_PT
dc.relationNovel molecularly imprinted photonic sensors applied to Inflammatory Bowel Diseases detection
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0925400520302951?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPhotonic sensorpt_PT
dc.subjectPhotonic crystalpt_PT
dc.subjectMolecularly-imprinted polymerpt_PT
dc.subjectFibrinopeptide Bpt_PT
dc.subjectVenous thromboembolism biomarkerpt_PT
dc.titleA biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarkerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNovel molecularly imprinted photonic sensors applied to Inflammatory Bowel Diseases detection
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F139634%2F2018/PT
oaire.citation.startPage127947pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume312pt_PT
oaire.fundingStreamPOR_NORTE
person.familyNameResende
person.familyNameFrasco
person.familyNameFerreira Sales
person.givenNameSara
person.givenNameManuela
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-id421F-CD9B-986A
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0002-3733-611X
person.identifier.orcid0000-0002-1813-8089
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridABA-7709-2020
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id57193727027
person.identifier.scopus-author-id9633427100
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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