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In Vitro Evaluation of the Photoreactivity and Phototoxicity of Natural Polyphenol Antioxidants

2021Journal article Open access
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dc.contributor.authorAguiar, Brandon
dc.contributor.authorCarmo, Helena
dc.contributor.authorGarrido, Jorge
dc.contributor.authorSousa Lobo, José M.
dc.contributor.authorAlmeida, Isabel F.
dc.date.accessioned2023-02-15T12:25:16Z
dc.date.available2023-02-15T12:25:16Z
dc.date.issued2021
dc.description.abstractPolyphenols are a large family of natural compounds widely used in cosmetic products due to their antioxidant and anti-inflammatory beneficial properties and their ability to prevent UV radiation-induced oxidative stress. Since these compounds present chromophores and are applied directly to the skin, they can react with sunlight and exert phototoxic effects. The available scientific information on the phototoxic potential of these natural compounds is scarce, and thus the aim of this study was to evaluate the photoreactivity and phototoxicity of five phenolic antioxidants with documented use in cosmetic products. A standard ROS assay was validated and applied to screen the photoreactivity of the natural phenolic antioxidants caffeic acid, ferulic acid, p-coumaric acid, 3,4-dihydroxyphenylacetic acid (DOPAC), and rutin. The phototoxicity potential was determined by using a human keratinocyte cell line (HaCaT), based on the 3T3 Neutral Red Uptake phototoxicity test. Although all studied phenolic antioxidants absorbed UV/Vis radiation in the range of 290 to 700 nm, only DOPAC was able to generate singlet oxygen. The generation of reactive oxygen species is an early-stage chemical reaction as part of the phototoxicity mechanism. Yet, none of the studied compounds decreased the viability of keratinocytes after irradiation, leading to the conclusion that they do not have phototoxic potential. The data obtained with this work suggests that these compounds are safe when incorporated in cosmetic products.pt_PT
dc.description.sponsorshipThis work is financed by national funds from FCT-Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020, UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences-UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB and UIDB/00081/2020 grant funded by FCT/MCTES (PIDDAC).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/molecules27010189pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/22298
dc.language.isoengpt_PT
dc.publisherMDPIpt_PT
dc.relationLA/P/0140/2020pt_PT
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationChemistry Research Unit of University of Porto
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/27/1/189pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPhotosafetypt_PT
dc.subjectPhotoreactivitypt_PT
dc.subjectPhototoxicitypt_PT
dc.subjectPolyphenolspt_PT
dc.subjectNatural phenolic antioxidantspt_PT
dc.subjectReactive oxygen speciespt_PT
dc.subjectKeratinocytespt_PT
dc.subjectSkin carept_PT
dc.subjectCosmetic productspt_PT
dc.titleIn Vitro Evaluation of the Photoreactivity and Phototoxicity of Natural Polyphenol Antioxidantspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleChemistry Research Unit of University of Porto
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00081%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage189pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume27pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGarrido
person.givenNameJorge
person.identifier.orcid0000-0001-8981-231X
person.identifier.scopus-author-id7202779733
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryf548e711-c68e-4c49-9471-9923c047116d
relation.isProjectOfPublicationfb6a6ebd-8625-4cef-b25e-e419945b33bc
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