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A passive direct methanol fuel cell as transducer of an electrochemical sensor, applied to the detection of carcinoembryonic antigen

2021Journal article Restricted access
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dc.contributor.authorCarneiro, Liliana P.T.
dc.contributor.authorFerreira, Nádia S.
dc.contributor.authorTavares, Ana P.M.
dc.contributor.authorPinto, Alexandra M.F.R.
dc.contributor.authorMendes, Adélio
dc.contributor.authorSales, Maria Goreti Ferreira
dc.date.accessioned2021-09-27T13:34:55Z
dc.date.embargo2031-12
dc.date.issued2021
dc.description.abstractThis work describes an electrochemical sensor with a biomimetic plastic antibody film for carcinoembryonic antigen (CEA, an important biomarker in colorectal cancer), integrated in the electrical circuit of a direct methanol fuel cell (DMFC), working in passive mode and used herein as power supply and signal transducer. In detail, the sensing layer for CEA consisted of a Fluorine-doped Tin Oxide (FTO) conductive glass substrate – connected to the negative pole side of the DMFC – with a conductive poly (3,4-ethylenedioxythiophene) (PEDOT) layer and a polypyrrol (PPy) molecularly-imprinted polymer (MIP), assembled in-situ. This sensing element is then closed using a cover FTO-glass, hold in place with a clip, connected to the positive side of the DMFC. When compared with control DMFCs, the power curves of DMFC/Sensor integrated system showed decreased power values due to the MIP layer interfaced in the electrical circuit, also displaying high stability signals. The DMFC/Sensor was further calibrated at room temperature, in different medium (buffer, a synthetic physiological fluid model and Cormay® serum), showing linear responses over a wide concentration range, with a limit of detection of 0.08 ng/mL. The DMFC/Sensor presented sensitive data, with linear responses from 0.1 ng/mL to 100 μg/mL and operating well in the presence of human serum. Overall, the results obtained evidenced the possibility of using a DMFC as a transducing element in an electrochemical sensor, confirming the sensitive and selective readings of the bio (sensing) imprinted film. This integration paves the way towards fully autonomous electrochemical devices, in which the integration of the sensor inside the fuel cell may be a subsequent direction.pt_PT
dc.description.sponsorshipThe authors gratefully acknowledge the financial support of the European Commission (FET-Open/H2020-Symbiotic/GA665046). Liliana Carneiro (Grant reference SFRH/BD/122954/2016) and Nádia Ferreira (Grant reference SFRH/BD/122955/2016) acknowledge the financial support from Fundação para a Ciência e Tecnologia (FCT). FCT is also acknowledged through reference UID/EMS/00532/2019.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bios.2020.112877pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/18565
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationFET-Open/H2020-Symbiotic/GA665046pt_PT
dc.relationINNOVATIVE AUTONOMOUS ELECTRICAL BIOSENSOR SYNERGISTICALLY ASSEMBLED INSIDE A PASSIVE DIRECT METHANOL FUEL CELL FOR SCREENING CANCER BIOMARKERS
dc.relationA new vision for fuel cells: application in cancer biomarker screening
dc.relationAutonomous biosensor for screening cancer biomarkers using passive Direct Methanol Fuel Cells
dc.relationStudy Center of Transport Phenomena
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0956566320308629?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectDirect methanol fuel cellpt_PT
dc.subjectSensorpt_PT
dc.subjectMolecularly-imprinted polymerpt_PT
dc.subjectCarcinoembryonic antigenpt_PT
dc.subjectPassive DMFCpt_PT
dc.titleA passive direct methanol fuel cell as transducer of an electrochemical sensor, applied to the detection of carcinoembryonic antigenpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINNOVATIVE AUTONOMOUS ELECTRICAL BIOSENSOR SYNERGISTICALLY ASSEMBLED INSIDE A PASSIVE DIRECT METHANOL FUEL CELL FOR SCREENING CANCER BIOMARKERS
oaire.awardTitleA new vision for fuel cells: application in cancer biomarker screening
oaire.awardTitleAutonomous biosensor for screening cancer biomarkers using passive Direct Methanol Fuel Cells
oaire.awardTitleStudy Center of Transport Phenomena
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/665046/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F122954%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F122955%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FEMS%2F00532%2F2019/PT
oaire.citation.startPage112877pt_PT
oaire.citation.titleBiosensors and Bioelectronicspt_PT
oaire.citation.volume175pt_PT
oaire.fundingStreamH2020
oaire.fundingStreamPOR_NORTE
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFerreira Sales
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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