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Plastic Antibody of Polypyrrole/Multiwall Carbon Nanotubes on Screen-Printed Electrodes for Cystatin C Detection

2021Journal article Open access
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dc.contributor.authorGomes, Rui S.
dc.contributor.authorGomez-Rodríguez, Blanca Azucena
dc.contributor.authorFernandes, Ruben
dc.contributor.authorSales, Goreti
dc.contributor.authorMoreira, Felismina
dc.contributor.authorDutra, Rosa F.
dc.date.accessioned2021-09-27T11:13:46Z
dc.date.available2021-09-27T11:13:46Z
dc.date.issued2021
dc.description.abstractThis work reports the design of a novel plastic antibody for cystatin C (Cys-C), an acute kidney injury biomarker, and its application in point-of-care (PoC) testing. The synthetic antibody was obtained by tailoring a molecularly imprinted polymer (MIP) on a carbon screen-printed electrode (SPE). The MIP was obtained by electropolymerizing pyrrole (Py) with carboxylated Py (Py-COOH) in the presence of Cys-C and multiwall carbon nanotubes (MWCNTs). Cys-C was removed from the molecularly imprinted poly(Py) matrix (MPPy) by urea treatment. As a control, a non-imprinted poly(Py) matrix (NPPy) was obtained by the same procedure, but without Cys-C. The assembly of the MIP material was evaluated in situ by Raman spectroscopy and the binding ability of Cys-C was evaluated by the cyclic voltammetry (CV) and differential pulse voltammetry (DPV) electrochemical techniques. The MIP sensor responses were measured by the DPV anodic peaks obtained in the presence of ferro/ferricyanide. The peak currents decreased linearly from 0.5 to 20.0 ng/mL of Cys-C at each 20 min successive incubation and a limit of detection below 0.5 ng/mL was obtained at pH 6.0. The MPPy/SPE was used to analyze Cys-C in spiked serum samples, showing recoveries <3%. This device showed promising features in terms of simplicity, cost and sensitivity for acute kidney injury diagnosis at the point of care.pt_PT
dc.description.sponsorshipThis research was funded by POCTEP/FEDER, grant number 0624_2IQBIONEURO_6_E”, by CNPQ (National Council for Scientific and Technological Development, Brazil), grant number 440605/2016-4 and by FCT (Fundação para a Ciência e Tecnologia, Portugal) with the Grant number SAICT-POL/24325/2016.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/bios11060175pt_PT
dc.identifier.issn2079-6374
dc.identifier.urihttp://hdl.handle.net/10400.22/18561
dc.language.isoengpt_PT
dc.publisherMDPIpt_PT
dc.relation0624_2IQBIONEURO_6_Ept_PT
dc.relation440605/2016-4pt_PT
dc.relationEarly detection and monitoring of metabolic progression of type 2 diabetes
dc.relation.publisherversionhttps://www.mdpi.com/2079-6374/11/6/175pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCystatin Cpt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectElectrochemical biosensorpt_PT
dc.subjectPolypyrenept_PT
dc.subjectMultiwall carbon nanotubespt_PT
dc.subjectAcute kidney injurypt_PT
dc.titlePlastic Antibody of Polypyrrole/Multiwall Carbon Nanotubes on Screen-Printed Electrodes for Cystatin C Detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEarly detection and monitoring of metabolic progression of type 2 diabetes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9876 - Politécnicos/SAICT-POL%2F24325%2F2016/PT
oaire.citation.issue6pt_PT
oaire.citation.startPage175pt_PT
oaire.citation.titleBiosensorspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream9876 - Politécnicos
person.familyNameFerreira Sales
person.familyNameMoreira
person.givenNameMaria Goreti
person.givenNameFelismina
person.identifier826970
person.identifier1589429
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.ciencia-idC917-6A46-A270
person.identifier.orcid0000-0001-9936-7336
person.identifier.orcid0000-0003-4237-8952
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id7005174717
person.identifier.scopus-author-id23486193000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublication.latestForDiscoveryb6d3a2d0-608a-4a92-834b-8d7b29069fef
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relation.isProjectOfPublication.latestForDiscovery1c00aa9e-809e-4831-ac65-e5c026e6e30f

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