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Abstract(s)
A necessidade de desenvolver técnicas de diagnóstico rápidas e de baixo custo é de extrema importância, principalmente quando se trata de melhorar as condições de saúde global. Um fator importante para a prevenção e tratamento das doenças neurodegenerativas é o seu correto diagnóstico precoce. Os biossensores são uma ferramenta que poderão ser muito úteis na construção de testes em contexto point-of-care, sendo estes dispositivos de rápida resposta, de custo reduzido, minimamente invasivos e de alta sensibilidade e especificidade. O principal objetivo deste trabalho visa o desenvolvimento de um biossensor eletroquímico, para o rastreio de doenças neurodegenerativas associadas ao envelhecimento. Tem como base a deteção de um biomarcador relacionado com os processos neuro-inflamatórios, a Interleucina 6 (IL-6). Para este efeito, são desenvolvidos polímeros de impressão molecular, à base de materiais naturais, para o reconhecimento desta proteína, nos quais a estrutura polimérica cresce diretamente sobre a área condutora de um elétrodo. O biossensor é avaliado do ponto de vista de sensibilidade, reprodutibilidade e seletividade, tendo em conta uma deteção eletroquímica na presença deste biomarcador.
The need to develop rapid and cost-effective diagnostic techniques is extremely important, especially when it comes to improving global health conditions. An important factor in the prevention and treatment of neurodegenerative diseases is their correct diagnosis. Biosensors are a tool that can prove very useful in the development of pointof-care tests, as these devices are minimally invasive and highly sensitive and specific. The main goal of this work is to develop an electrochemical biosensor for screening of neurodegenerative diseases associated with ageing. It is based on the detection of a biomarker related to neuroinflammatory processes, Interleukin 6 (IL-6). For this purpose, molecularly imprinted polymer (MIP) of natural origin is being developed to detect the protein, with the polymeric structure growing directly on the conductive area of the carbon-screen printed electrode. The biosensor is evaluated in terms of sensitivity, reproducibility, and selectivity, with regard the electrochemical detection of the presence of the biomarker.
The need to develop rapid and cost-effective diagnostic techniques is extremely important, especially when it comes to improving global health conditions. An important factor in the prevention and treatment of neurodegenerative diseases is their correct diagnosis. Biosensors are a tool that can prove very useful in the development of pointof-care tests, as these devices are minimally invasive and highly sensitive and specific. The main goal of this work is to develop an electrochemical biosensor for screening of neurodegenerative diseases associated with ageing. It is based on the detection of a biomarker related to neuroinflammatory processes, Interleukin 6 (IL-6). For this purpose, molecularly imprinted polymer (MIP) of natural origin is being developed to detect the protein, with the polymeric structure growing directly on the conductive area of the carbon-screen printed electrode. The biosensor is evaluated in terms of sensitivity, reproducibility, and selectivity, with regard the electrochemical detection of the presence of the biomarker.
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Keywords
Biossensor Doença de Alzheimer Interleucina 6 Sensor eletroquímico Polímero de impressão molecular Biosensor Alzheimer's disease Interleukin 6 Electrochemical sensor Molecularly imprinted polymer