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Fuel cells operating as an immunosensor for cancer biomarker screening

2023Journal article Restricted access
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dc.contributor.authorFerreira, Nadia S.
dc.contributor.authorCarneiro, Liliana P.T
dc.contributor.authorPinto, Alexandra M.F.R.
dc.contributor.authorSales, M. Goreti F.
dc.date.accessioned2024-04-18T14:24:07Z
dc.date.available2024-04-18T14:24:07Z
dc.date.issued2023
dc.description.abstractThis work presents the first integration of antibodies into one of the electrodes of a passive direct methanol fuel cell (DMFC) to create an autonomous immunosensor for cancer antigen 15–3 (CA15-3). The anode of the fuel cell was first made of carbon cloth with carbon black, platinum and ruthenium nanoparticles and then modified with anti-CA15-3, while the cathode was made of carbon cloth with carbon black and platinum nanoparticles. This hybrid DMFC (hDMFC), which works as an immunosensor, was fed with dilute methanol to generate a concentration-dependent current. The hDMFC device was calibrated in different media (buffer and serum), by incubating increasing concentrations of CA15-3 standard solutions, ranging from 47 to 750 U/mL. The linear response ranged from 188 to 563 U/mL, and the limit of detection (LoD) was 39.8 U/mL. The selectivity of the biosensor was also evaluated with competing cancer biomarkers, such as carcinoembryonic antigen (CEA), and cancer antigen 125 (CA-125), as well as with other compounds normally present in blood plasma (ascorbic acid, glucose, uric acid and urea), adjusted to the normal range of concentrations in serum. The results obtained indicate good selectivity of the immunosensor in the fuel cell. In general, the immunosensor showed a good response considering its integration into a passive DMFC. It is a good sensor for point-of-care (PoC) diagnosis because it has a good performance in human serum and, most importantly, it pursues electrical autonomy.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNádia S. Ferreira, Liliana P.T. Carneiro, Alexandra M.F.R. Pinto, M. Goreti F. Sales, Fuel cells operating as an immunosensor for cancer biomarker screening, Biosensors and Bioelectronics: X, Volume 14, 2023, 100344, ISSN 2590-1370, https://doi.org/10.1016/j.biosx.2023.100344.pt_PT
dc.identifier.doi10.1016/j.biosx.2023.100344pt_PT
dc.identifier.issn2590-1370
dc.identifier.urihttp://hdl.handle.net/10400.22/25362
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationFET-Open/H2020-Symbiotic/GA665046pt_PT
dc.relationSFRH/BD/122955/2016pt_PT
dc.relationUIDB/00532/2021pt_PT
dc.relationUIDP/00532/2021pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2590137023000419pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectImmunosensorpt_PT
dc.subjectDirect methanol fuel cellpt_PT
dc.subjectCA15-3pt_PT
dc.subjectCancer biomarkerpt_PT
dc.subjectPoint-of-carept_PT
dc.titleFuel cells operating as an immunosensor for cancer biomarker screeningpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBiosensors and Bioelectronics: Xpt_PT
oaire.citation.volume14pt_PT
person.familyNameSilva Ferreira
person.familyNameTeixeira Carneiro
person.familyNameFerreira Rodrigues Pinto
person.givenNameNadia
person.givenNameLiliana Patrícia
person.givenNameAlexandra
person.identifier.ciencia-id4310-FE12-C862
person.identifier.ciencia-id011B-41E0-E059
person.identifier.ciencia-id7518-FD35-9DFD
person.identifier.orcid0000-0001-8251-1223
person.identifier.orcid0000-0002-2937-5186
person.identifier.scopus-author-id55888120300
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication206c7b33-eac7-4b9f-be44-1072cbcb5c26
relation.isAuthorOfPublicationa522b9a5-f365-497b-9d30-d54a426d406e
relation.isAuthorOfPublicationea0c8b9a-8fba-4d53-a9fe-6275e978e55d
relation.isAuthorOfPublication.latestForDiscoveryea0c8b9a-8fba-4d53-a9fe-6275e978e55d

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