Publication
Electrochemical biosensor based on biomimetic material for myoglobin detection
| dc.contributor.author | Moreira, Felismina T. C. | |
| dc.contributor.author | Dutra, Rosa A.F. | |
| dc.contributor.author | Noronha, João P. C. | |
| dc.contributor.author | Sales, M. Goreti F. | |
| dc.date.accessioned | 2015-10-16T12:11:55Z | |
| dc.date.available | 2015-10-16T12:11:55Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | A novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVCsingle bondCOOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FTIR) studies confirmed the surface modification. The MIP/Au-SPE devices displayed a linear behaviour in EIS from 0.852 to 4.26 μg mL−1, of positive slope 6.50 ± 1.48 (kΩ mL μg−1). The limit of detection was 2.25 μg mL−1. Square wave voltammetric (SWV) assays were made in parallel and showed linear responses between 1.1 and 2.98 μg mL−1. A current decrease was observed against Myo concentration, producing average slopes of −0.28 ± 0.038 μA mL μg−1. MIP/Au-SPE also showed good results in terms of selectivity. The error% found for each interfering species were 7% for troponin T (TnT), 11% for bovine serum albumin (BSA) and 2% for creatine kinase MB (CKMB), respectively. Overall, the technical modification over the Au-SPE was found a suitable approach for screening Myo in biological fluids. | pt_PT |
| dc.identifier.doi | 10.1016/j.electacta.2013.06.061 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/6729 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0013468613011675 | pt_PT |
| dc.subject | Biosensor | pt_PT |
| dc.subject | Surface molecular imprint | pt_PT |
| dc.subject | Screen printed electrodes | pt_PT |
| dc.subject | Sensor | pt_PT |
| dc.subject | Myoglobin | pt_PT |
| dc.title | Electrochemical biosensor based on biomimetic material for myoglobin detection | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 487 | pt_PT |
| oaire.citation.startPage | 481 | pt_PT |
| oaire.citation.title | Electrochimica Acta | pt_PT |
| oaire.citation.volume | 107 | pt_PT |
| person.familyName | Moreira | |
| person.givenName | Felismina | |
| person.identifier | 1589429 | |
| person.identifier.ciencia-id | C917-6A46-A270 | |
| person.identifier.orcid | 0000-0003-4237-8952 | |
| person.identifier.scopus-author-id | 23486193000 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 9c13153d-a73a-49ff-b7f6-c9733bc6725e | |
| relation.isAuthorOfPublication.latestForDiscovery | 9c13153d-a73a-49ff-b7f6-c9733bc6725e |