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Modulation of Siderophore Production by Pseudomonas fluorescens Through the Manipulation of the Culture Medium Composition

2020Journal article Open access
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dc.contributor.authorVindeirinho, João M.
dc.contributor.authorSoares, Helena M.V.M.
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2022-03-23T17:08:54Z
dc.date.available2025-01-01T01:30:48Z
dc.date.issued2020
dc.description.abstractPseudomonas fluorescens has the ability to produce the siderophore pyoverdine, a biotechnologically significant iron chelator, which has a wide range of potential applications, such as in agriculture (iron fertilizers) and medicine (development of antibiotics). The present work aimed to evaluate the influence of culture medium composition on the production of siderophores by P. fluorescens DSM 50090, an industrial relevant strain. It was found that the bacterium grown in minimal medium succinate (MMS) had a higher siderophore production than in King B medium. The replacement of succinate by glycerol or dextrose, in minimal medium, originated lower siderophore production. The increase of succinate concentration, the addition of amino acids or the reduction of phosphate in the culture medium did not improve siderophore production by P. fluorescens. The results obtained strongly suggest that (i) MMS is more appropriate than King B for large-scale production of siderophores; (ii) the modification of the culture medium composition, particularly the type of carbon source, influences the level of siderophore secreted; (iii) the production of siderophore by P. fluorescens seems to be a tightly regulated process; once a maximum siderophore concentration has been reached in the culture medium, the bacterium seems to be unable to produce more compoundpt_PT
dc.description.sponsorshipJoão M. Vindeirinho received the grant from the project PTDC-AGR-TEC/0458/2014—POCI-01-0145-FEDER-016681. This work is financed by the FEDER funds through the Operational Competitiveness Factors Program—COMPETE and by national funds through FCT—Foundation for Science and Technology within the scope of the project PTDC-AGR-TEC/0458/2014—POCI-01-0145-FEDER-016681.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVindeirinho, J.M., Soares, H.M. & Soares, E.V. Modulation of Siderophore Production by Pseudomonas fluorescens Through the Manipulation of the Culture Medium Composition. Appl Biochem Biotechnol 193, 607–618 (2021). https://doi.org/10.1007/s12010-020-03349-zpt_PT
dc.identifier.doi10.1007/s12010-020-03349-zpt_PT
dc.identifier.issn10.1007/s12010-020-03349-z
dc.identifier.urihttp://hdl.handle.net/10400.22/20302
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationPTDC-AGR-TEC/0458/2014—POCI-01-0145-FEDER-016681pt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s12010-020-03349-zpt_PT
dc.subjectCulture medium optimizationpt_PT
dc.subjectBacterial nutritionpt_PT
dc.subjectPseudomonas fluorescenspt_PT
dc.subjectSiderophorespt_PT
dc.subjectPyoverdinept_PT
dc.titleModulation of Siderophore Production by Pseudomonas fluorescens Through the Manipulation of the Culture Medium Compositionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage618pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage607pt_PT
oaire.citation.titleApplied Biochemistry and Biotechnologypt_PT
oaire.citation.volume193pt_PT
person.familyNameSoares
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id7102060173
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219

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