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Broadband spectral verification of optical clearing reversibility in lung tissue

2022-08-16Journal article Restricted access
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dc.contributor.authorOliveira, Luís R.
dc.contributor.authorFerreira, Ricardo M.
dc.contributor.authorPinheiro, Maria R.
dc.contributor.authorSilva, Hugo F.
dc.contributor.authorTuchin, Valery V.
dc.contributor.authorOliveira, Luís
dc.date.accessioned2023-04-03T11:27:33Z
dc.date.embargo2035-12-31
dc.date.issued2022-08-16
dc.description.abstractThe increase of tissue transparency through sequential optical immersion clearing treatments and treatment reversibility have high interest for clinical applications. To evaluate the clearing reversibility in a broad spectral range and the magnitude of the transparency created by a second treatment, the present study consisted on measuring the spectral collimated transmittance of lung tissues during a sequence of two treatments with electronic cigarette (e-cig) fluid, which was intercalated with an immersion in saline. The saline immersion clearly reverted the clearing effect in the lung tissue in the spectral range between 220 and 1000 nm. By a later application of a second treatment with the e-cig fluid, the magnitude of the optical clearing effect was observed to be about the double as the one observed in the first treatment, showing that the molecules of the optical clearing agent might have converted some bound water into mobile water during the first treatment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationOliveira, L. R., Ferreira, R. M., Pinheiro, M. R., Silva, H. F., Tuchin, V. V., Oliveira, L. M., J. Biophotonics 2022, e202200185. https://doi.org/10.1002/jbio.202200185pt_PT
dc.identifier.doi10.1002/jbio.202200185pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/22658
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1002/jbio.202200185pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectElectronic cigarettept_PT
dc.subjectImmersion tissue optical clearingpt_PT
dc.subjectOptical clearing agentpt_PT
dc.subjectOptical clearing efficiencypt_PT
dc.subjectPropylene glycolpt_PT
dc.subjectRed blood cellspt_PT
dc.subjectRefractive indexpt_PT
dc.subjectCollimated transmittancept_PT
dc.titleBroadband spectral verification of optical clearing reversibility in lung tissuept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.titleJournal of Biophotonicspt_PT
oaire.citation.volume16pt_PT
person.familyNameOliveira
person.givenNameLuís
person.identifier414820
person.identifier.ciencia-idC312-F342-BD23
person.identifier.orcid0000-0003-0667-3428
person.identifier.ridB-7198-2017
person.identifier.scopus-author-id55853723900
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4e2de4d7-142a-400f-8dbf-cd0ff9551093
relation.isAuthorOfPublication.latestForDiscovery4e2de4d7-142a-400f-8dbf-cd0ff9551093

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