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Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers

2020Journal article Open access
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dc.contributor.authorPiloto, Ana Margarida L.
dc.contributor.authorRibeiro, David S.M.
dc.contributor.authorRodrigues, S. Sofia M.
dc.contributor.authorSantos, João L.M.
dc.contributor.authorFerreira Sales, Maria Goreti
dc.date.accessioned2022-01-14T11:31:55Z
dc.date.available2022-01-14T11:31:55Z
dc.date.issued2020
dc.description.abstractHerein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIT), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe@MPA QDs is quenched in a concentration dependent manner. Using surface imprinting technology, the optimal fluorescence signals yielded a linear response versus logarithm of IL-2 concentration from 35 fg/ml to 39 pg/ml, in a 1000-fold diluted synthetic human serum. The limit of detection obtained is 5.91 fg/ml, lying below the concentration levels of IL-2 with clinical interest for cancer diagnosis (9.4–19.2 pg/ml). Overall, the method presented herein is a demonstration that the combination of MIP and QDs for protein detection constitutes a powerful tool in clinical analysis, providing low cost, sensitive and quick responses. The same concept may be further extended to other proteins of interest.pt_PT
dc.description.sponsorshipThe authors acknowledge the financial support of European Research Council though the Starting Grant, 3P’s Starting Grant/ERC (GA 311086)pt_PT
dc.description.versioninfo:eu-repo/semantics/submittedVersionpt_PT
dc.identifier.citationPiloto AML, Ribeiro DSM, Rodrigues SSM, Santos JLM, Ferreira Sales MG, Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers, Sensors and Actuators: B. Chemical (2019), doi: https://doi.org/10.1016/j.snb.2019.127343pt_PT
dc.identifier.doi10.1016/j.snb.2019.127343pt_PT
dc.identifier.issn0925-4005
dc.identifier.urihttp://hdl.handle.net/10400.22/19485
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0925400519315424pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectQuantum dotspt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectConjugated-Qdspt_PT
dc.subjectProteinpt_PT
dc.subjectInterleukin-2pt_PT
dc.subjectCancer biomarkerpt_PT
dc.titleLabel-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymerspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage127343pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume304pt_PT
person.familyNameL. Piloto Cerqueira
person.familyNameFerreira Sales
person.givenNameAna Margarida
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-idAD1C-28E2-F781
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0002-2374-2467
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridG-5788-2018
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id8527885100
person.identifier.scopus-author-id7005174717
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbed0bc46-bcdd-4f89-8eed-ac6c7620029e
relation.isAuthorOfPublicationb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isAuthorOfPublication.latestForDiscoverybed0bc46-bcdd-4f89-8eed-ac6c7620029e

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