Repository logo
 
Publication

Enhancing extraction and purification of phycocyanin from Arthrospira sp. with lower energy consumption

dc.contributor.authorGorgich, Malihe
dc.contributor.authorPassos, Marieta L.C.
dc.contributor.authorMata, Teresa M.
dc.contributor.authorMartins, António A.
dc.contributor.authorSaraiva, M. Lúcia M.F.S.
dc.contributor.authorCaetano, Nídia
dc.date.accessioned2021-09-24T13:48:41Z
dc.date.available2021-09-24T13:48:41Z
dc.date.issued2020
dc.description.abstractPhycocyanin is a light-blue water-soluble natural pigment with wide application in fields including pharmaceutical, cosmetic and food industry. Arthrospira platensis is considered a natural bio-factory for obtaining phycocyanin, and although suitable cultivation conditions can enhance the pigment content, the importance of extraction and purification should not be overlooked, because the chemicals used in those steps can affect the quality of phycocyanin. Further, changes in temperature, or approaches used to extract other protein types from biomass, have been reported to degrade phycocyanin, influencing its final quality, while also contributing to higher energy consumption in the extraction process. Hence, this work compares several approaches for phycocyanin extraction, and analyzed the effects of changes in operating conditions, such as exposure time, application of solvent buffer, and ultrasonication, on the extraction yield, the extracts purity, and the process energy use efficiency. Experimental results point to promising improvements in the extraction yield with high purity of phycocyanin (44.24 mg/g with purity ratio of 0.34), when suitable operating parameters were used (2 h exposure to potassium phosphate buffer with 20 min of ultrasonication).pt_PT
dc.description.sponsorshipThis work was financially supported by: Base Funding — UIDB/00511/2020 of Laboratory for Process Engineering, Environment, Biotechnology and Energy, Portugal — LEPABE — funded by National funds through the FCT/MCTES (PIDDAC); Base Funding — UIDB/04730/2020 of Center for Innovation in Engineering and Industrial Technology, CIETI — funded by national funds through the FCT/MCTES (PIDDAC); project EXTRATOTECA -POCI-01-0247-FEDER-033784, funded by FEDER funds through COMPETE2020 — Programa Operacional Competitividade e Internacionalização (POCI) and by national funds (PIDDAC) through FCT/MCTES; Base Funding - UIDB/QUI/50006/2020 of Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos; project Tailored NanoGumbos: The green key to wound infections chemsensing, funded by Portugal 2020, by European Regional Development Fund (FEDER) through Operational Competitiveness Program (COMPETE) - reference POCI-01-0145-FEDER-030163. António Martins and Marieta Passos thank FCT (Fundação para a Ciência e Tecnologia) for funding through program DL 57/2016 – Norma transitória .pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.egyr.2020.11.151pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/18537
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2352484720315766pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectArthrospira platensispt_PT
dc.subjectExtractionpt_PT
dc.subjectPhycocyaninpt_PT
dc.subjectPhycobiliproteinpt_PT
dc.subjectPurificationpt_PT
dc.subjectSolvent-bufferpt_PT
dc.titleEnhancing extraction and purification of phycocyanin from Arthrospira sp. with lower energy consumptionpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157419/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157604/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157669/PT
oaire.citation.endPage318pt_PT
oaire.citation.startPage312pt_PT
oaire.citation.titleEnergy Reportspt_PT
oaire.citation.volume6pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCaetano
person.givenNameNídia
person.identifierR-000-DJC
person.identifier.ciencia-id1F1D-73E2-BFBF
person.identifier.orcid0000-0002-2185-6401
person.identifier.ridI-3934-2012
person.identifier.scopus-author-id55901684900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication4bbb44e7-228a-48fb-abc2-8df5e27d0b48
relation.isAuthorOfPublication.latestForDiscovery4bbb44e7-228a-48fb-abc2-8df5e27d0b48
relation.isProjectOfPublicationafea681e-0b1c-4fdc-abf5-a3ce4735ff6f
relation.isProjectOfPublication23ff3139-1d2c-416e-ad3e-3f2e54271bef
relation.isProjectOfPublicationf0e57b94-d574-42d2-b8f3-5780816fcd7c
relation.isProjectOfPublication.latestForDiscoveryafea681e-0b1c-4fdc-abf5-a3ce4735ff6f

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
COM_CIETI_NSC_2020.pdf
Size:
492.29 KB
Format:
Adobe Portable Document Format