Repository logo
 
Publication

Metal(loid) oxide (Al2O3, Mn3O4, SiO2 and SnO2) nanoparticles cause cytotoxicity in yeast via intracellular generation of reactive oxygen species

2019Journal article Restricted access
Simple item page
dc.contributor.authorSousa, Cátia A.
dc.contributor.authorSoares, Helena M. V. M.
dc.contributor.authorSoares, Eduardo
dc.date.accessioned2021-02-26T11:32:28Z
dc.date.embargo2120
dc.date.issued2019
dc.description.abstractIn this work, the physicochemical characterization of five (Al2O3, In2O3, Mn3O4, SiO2 and SnO2) nanoparticles (NPs) was carried out. In addition, the evaluation of the possible toxic impacts of these NPs and the respective modes of action were performed using the yeast Saccharomyces cerevisiae. In general, in aqueous suspension, metal(loid) oxide (MOx) NPs displayed an overall negative charge and agglomerated; these NPs were practically insoluble (dissolution < 8%) and did not generate detectable amounts of reactive oxygen species (ROS) under abiotic conditions. Except In2O3 NPs, which did not induce an obvious toxic effect on yeast cells (up to 100 mg/L), the other NPs induced a loss of cell viability in a dose-dependent manner. The comparative analysis of the loss of cell viability induced by the NPs with the ions released by NPs (NPs supernatant) suggested that SiO2 toxicity was mainly caused by the NPs themselves, Al2O3 and SnO2 toxic effects could be attributed to both the NPs and the respective released ions and Mn3O4 harmfulness could be mainly due to the released ions. Al2O3, Mn3O4, SiO2 and SnO2 NPs induced the loss of metabolic activity and the generation of intracellular ROS without permeabilization of plasma membrane. The co-incubation of yeast cells with MOx NPs and a free radical scavenger (ascorbic acid) quenched intracellular ROS and significantly restored cell viability and metabolic activity. These results evidenced that the intracellular generation of ROS constituted the main cause of the cytotoxicity exhibited by yeasts treated with the MOx NPs. This study highlights the importance of a ROS-mediated mechanism in the toxicity induced by MOx NPs.pt_PT
dc.description.sponsorshipThis work was performed in the framework of the financing by Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte and LAQV (UID/QUI/50006/2019) with funding from FCT/MCTES through national funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00253-019-09903-ypt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/17176
dc.language.isoengpt_PT
dc.publisherSpringerpt_PT
dc.relationNORTE-01-0145-FEDER-000004pt_PT
dc.relationCentre of Biological Engineering - University of Minho
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00253-019-09903-ypt_PT
dc.subjectEsterase activitypt_PT
dc.subjectMembrane integritypt_PT
dc.subjectOxidative stresspt_PT
dc.subjectPhysicochemical properties of nanomaterialspt_PT
dc.subjectToxicitypt_PT
dc.titleMetal(loid) oxide (Al2O3, Mn3O4, SiO2 and SnO2) nanoparticles cause cytotoxicity in yeast via intracellular generation of reactive oxygen speciespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering - University of Minho
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIO%2F04469%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F50006%2F2019/PT
oaire.citation.endPage6269pt_PT
oaire.citation.issue15pt_PT
oaire.citation.startPage6257pt_PT
oaire.citation.titleApplied Microbiology and Biotechnologypt_PT
oaire.citation.volume103pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameA. Sousa
person.familyNameSoares
person.givenNameCátia
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-id4715-BD01-8460
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-3719-4454
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridS-2889-2016
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id55650545800
person.identifier.scopus-author-id7102060173
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0c464330-a218-49cd-92af-08056285cb74
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isProjectOfPublication249b9920-551c-474c-b43c-c674bd5c8dca
relation.isProjectOfPublicationf943d798-b7fe-4c3c-87a7-46b3e5ac86be
relation.isProjectOfPublication.latestForDiscovery249b9920-551c-474c-b43c-c674bd5c8dca

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART_CIETI_EVS-16_2019.pdf
Size:
850.29 KB
Format:
Adobe Portable Document Format
Download

Collections

ISEP – CIETI – Artigos
ISEP – DEQ – Artigos