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In-situ production of Histamine-imprinted polymeric materials for electrochemical monitoring of fish

2020Journal article Open access
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dc.contributor.authorSerrano, Verónica
dc.contributor.authorCardoso, Ana Rita
dc.contributor.authorDiniz, Mário
dc.contributor.authorSales, Maria Goreti Ferreira
dc.date.accessioned2021-09-27T14:26:28Z
dc.date.available2021-09-27T14:26:28Z
dc.date.issued2020
dc.description.abstractHistamine (HIS) is a major public health problem due to its toxic properties. High levels can cause a chronic toxicity as poisoning and can be used as a signal of food hygiene. Thus, a new electrochemical sensor for HIS detection in fish is presented herein, prepared by tailoring a molecularly imprinted polymer (MIP) sensing material on a gold screen-printed electrode (Au-SPEs), in which the polymeric film was generated in-situ. This film was obtained by electropolymerizing aniline under conditions that preserved the chemical structure of HIS. Raman spectroscopy followed the chemical changes occurring at each stage of the electrode modification. The device performance was assessed by evaluating the changes in electron transfer properties of a standard redox probe [Fe(CN)6]4−/[Fe(CN)6]3−, by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). EIS was also used to calibrate the sensor, being the standard solutions prepared under different background media (electrolyte or a blank sample of fish extract). The device displayed a linear response from 500 nM to 1 mM, with a limit of detection of 210 nM, and a selective behaviour against tyramine, another amine related to fish degradation. The sensing system was further employed to monitor the HIS content in samples in different time points of storage at ambient temperature. The obtained results were in agreement with the ELISA method, while offering more reproducible data. In general, the optimized sensor allowed reproducible and accurate analysis of fish samples subject to degradation and was completely assembled in-situ, in a very simple and straightforward approach. The device is low cost and suitable for further adaptation to on-site analysis, as required in food control.pt_PT
dc.description.sponsorshipThe authors acknowledge national funding to the National Foundation for Science and Technology (FCT), I.P., and European funding to FEDER (European Funding or Regional Development), via COMPETE2020 – POCI (operational program for internationalization and competitiveness), through projects PTDC/MAR-BIO/6044/2014 and PTDC/AAG-TEC/5400/2014.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2020.127902pt_PT
dc.identifier.issn09254005
dc.identifier.urihttp://hdl.handle.net/10400.22/18575
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0925400520302495?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectElectropolymerizationpt_PT
dc.subjectScreen-printed electrodept_PT
dc.subjectAnilinept_PT
dc.subjectHistaminept_PT
dc.subjectFishpt_PT
dc.titleIn-situ production of Histamine-imprinted polymeric materials for electrochemical monitoring of fishpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-BIO%2F6044%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FAAG-TEC%2F5400%2F2014/PT
oaire.citation.startPage127902pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume311pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
person.familyNameMorgado Serrano
person.familyNameCardoso
person.familyNameFerreira Sales
person.givenNameVerónica
person.givenNameAna Rita
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-id881B-B056-9618
person.identifier.ciencia-idF717-044B-1E8D
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0001-5605-3767
person.identifier.orcid0000-0002-8641-0865
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id57125522400
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery7952f19a-f207-4218-b3df-5aa5dbc49a9a
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