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Artificial antibodies for troponin T by its imprinting on the surface of multiwalled carbon nanotubes: Its use as sensory surfaces

dc.contributor.authorMoreira, Felismina T. C.
dc.contributor.authorDutra, Rosa A.F.
dc.contributor.authorNoronha, João P. C.
dc.contributor.authorCunha, Alexandre L.
dc.contributor.authorSales, Goreti
dc.date.accessioned2015-10-19T13:41:20Z
dc.date.available2015-10-19T13:41:20Z
dc.date.issued2011
dc.description.abstractA novel artificial antibody for troponin T (TnT) was synthesized by molecular imprint (MI) on the surface of multiwalled carbon nanotubes (MWCNT). This was done by attaching TnT to the MWCNT surface, and filling the vacant spaces by polymerizing under mild conditions acrylamide (monomer) in N,N′-methylenebisacrylamide (cross-linker) and ammonium persulphate (initiator). After removing the template, the obtained biomaterial was able to rebind TnT and discriminate it among other interfering species. Stereochemical recognition of TnT was confirmed by the non-rebinding ability displayed by non-imprinted (NI) materials, obtained by imprinting without a template. SEM and FTIR analysis confirmed the surface modification of the MWCNT. The ability of this biomaterial to rebind TnT was confirmed by including it as electroactive compound in a PVC/plasticizer mixture coating a wire of silver, gold or titanium. Anionic slopes of 50 mV decade−1 were obtained for the gold wire coated with MI-based membranes dipped in HEPES buffer of pH 7. The limit of detection was 0.16 μg mL−1. Neither the NI-MWCNT nor the MWCNT showed the ability to recognize the template. Good selectivity was observed against creatinine, sucrose, fructose, myoglobin, sodium glutamate, thiamine and urea. The sensor was tested successfully on serum samples. It is expected that this work opens new horizons on the design of new artificial antibodies for complex protein structures.pt_PT
dc.identifier.doi10.1016/j.bios.2011.07.026
dc.identifier.urihttp://hdl.handle.net/10400.22/6752
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0956566311004544pt_PT
dc.subjectArtificial antibodypt_PT
dc.subjectTroponin Tpt_PT
dc.subjectMolecularly imprinted biomaterialspt_PT
dc.subjectPotentiometric sensorpt_PT
dc.subjectCardiac biomarkerpt_PT
dc.titleArtificial antibodies for troponin T by its imprinting on the surface of multiwalled carbon nanotubes: Its use as sensory surfacespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage250pt_PT
oaire.citation.startPage243pt_PT
oaire.citation.titleBiosensors and Bioelectronicspt_PT
oaire.citation.volume28pt_PT
person.familyNameMoreira
person.familyNameFerreira Sales
person.givenNameFelismina
person.givenNameMaria Goreti
person.identifier1589429
person.identifier826970
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id23486193000
person.identifier.scopus-author-id7005174717
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublicationb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isAuthorOfPublication.latestForDiscovery9c13153d-a73a-49ff-b7f6-c9733bc6725e

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