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Chronic exposure of the freshwater alga Pseudokirchneriella subcapitata to five oxide nanoparticles: Hazard assessment and cytotoxicity mechanisms

2019Journal article Restricted access
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dc.contributor.authorSousa, Cátia A.
dc.contributor.authorSoares, Helena M.V.M.
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2021-02-26T10:33:34Z
dc.date.embargo2120
dc.date.issued2019
dc.description.abstractThe increasing use of nanoparticles (NPs) unavoidably enhances their unintended introduction into the aquatic systems, raising concerns about their nanosafety. This work aims to assess the toxicity of five oxide NPs (Al2O3, Mn3O4, In2O3, SiO2 and SnO2) using the freshwater alga Pseudokirchneriella subcapitata as a primary producer of ecological relevance. These NPs, in OECD medium, were poorly soluble and unstable (displayed low zeta potential values and presented the tendency to agglomerate). Using the algal growth inhibition assay and taking into account the respective 72 h-EC50 values, it was possible to categorize the NPs as: toxic (Al2O3 and SnO2); harmful (Mn3O4 and SiO2) and non-toxic/non-classified (In2O3). The toxic effects were mainly due to the NPs, except for SnO2 which toxicity can mainly be attributed to the Sn ions leached from the NPs. A mechanistic study was undertaken using different physiological endpoints (cell membrane integrity, metabolic activity, photosynthetic efficiency and intracellular ROS accumulation). It was observed that Al2O3, Mn3O4 and SiO2 induced an algistatic effect (growth inhibition without loss of membrane integrity) most likely as a consequence of the cumulative effect of adverse outcomes: i) reduction of the photosynthetic efficiency of the photosystem II (ФPSII); ii) intracellular ROS accumulation and iii) loss of metabolic activity. SnO2 NPs also provoked an algistatic effect probably as a consequence of the reduction of ФPSII since no modification of intracellular ROS levels and metabolic activity were observed. Altogether, the results here presented allowed to categorize the toxicity of the five NPs and shed light on the mechanisms behind NPs toxicity in the green alga P. subcapitata.pt_PT
dc.description.sponsorshipThis work was performed in the framework of the financing by Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte and LAQV (UID/QUI/50006/2013—POCI/01/0145/FEDER/007265) with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquatox.2019.105265pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/17174
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationUID/QUI/50006/2013—POCI/01/0145/FEDER/007265pt_PT
dc.relationCentre of Biological Engineering - University of Minho
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0166445X19303169?via%3Dihubpt_PT
dc.subjectMetabolic (esterase) activitypt_PT
dc.subjectMicroalgapt_PT
dc.subjectNanomaterialspt_PT
dc.subjectNanotoxicitypt_PT
dc.subjectOxidative stresspt_PT
dc.subjectPhotosynthesispt_PT
dc.titleChronic exposure of the freshwater alga Pseudokirchneriella subcapitata to five oxide nanoparticles: Hazard assessment and cytotoxicity mechanismspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering - University of Minho
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIO%2F04469%2F2019/PT
oaire.citation.startPage105265pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume214pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSoares
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id7102060173
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isProjectOfPublication249b9920-551c-474c-b43c-c674bd5c8dca
relation.isProjectOfPublication.latestForDiscovery249b9920-551c-474c-b43c-c674bd5c8dca

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