Repository logo
 
Publication

Paper-based (bio)sensor for label-free detection of 3-nitrotyrosine in human urine samples using molecular imprinted polymer

2020Journal article Open access
Simple item page
dc.contributor.authorMartins, Gabriela V.
dc.contributor.authorMarques, Ana C.
dc.contributor.authorFortunato, Elvira
dc.contributor.authorSales, Maria Goreti Ferreira
dc.date.accessioned2021-09-28T08:41:57Z
dc.date.available2021-09-28T08:41:57Z
dc.date.issued2020
dc.description.abstractOver the last years, paper technology has been widely spread as a more affordable, sustainable and reliable support material to be incorporated in the design of point-of-care (POC) diagnostic devices. However, the single work employing a paper-based device for 3-nitrotyrosine (3-NT), a relevant biomarker for oxidative stress (OS) that is a major origin for many diseases, is incapable of reading successfully complex samples because every species that oxidizes before ~0.75 V will also contribute to the final response. Thus, the introduction of a selective element was made into this set-up by including a molecularly-imprinted polymer (MIP) tailored in-situ. Herein, a novel MIP for 3-NT was assembled directly on a paper platform, made conductive with carbon ink and suitable for an electrochemical transduction. The biomimetic material was produced by electropolymerization of phenol after optimizing several experimental parameters, such a scan-rate, number of cycles, range of potential applied, monomer and template concentrations. Under optimal conditions, the label-free sensor was able to respond to 3-NT from 500 nM to 1 mM, yielding a limit of detection of 22.3 nM. Finally, the applicability of the (bio)sensor was tested by performing calibration assays in human urine samples and a good performance was obtained in terms of sensitivity, selectivity and reproducibility. Overall, the attributes of the herein described sensing approach can be compared to a very limited number of other electrochemical devices, that are still using a conventional three electrode system, making this paper-sustained device the first electrochemical (bio)sensor with potential to become a portable and low-cost diagnostic tool for 3-NT. In general, the incorporation of molecular imprinting technology coupled to electrochemical transduction enabled the fabrication of suitable smart sensors for wide screening approaches.pt_PT
dc.description.sponsorshipFundação para a Ciência e Tecnologia (FCT) supported this work through FEDER funds from COMPETE 2020 Program and National Funds, under the project PTDC/AAG-TEC/5400/2014 & POCI-01-0145-FEDER-016637, NORTE-01-0145-FEDER-024358, UID/CTM/50025/2019 and the PhD Grants references SFRH/BD/94159/2013 (GVM) and SFRH/BD/115173/2016 (ACM). H2020 also supported this work through the project MindGAP/FET-Open/GA829040.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.sbsr.2020.100333pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/18588
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-0145-FEDER-016637pt_PT
dc.relationNORTE-01-0145-FEDER-024358pt_PT
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationNanobiosensing platform based on MIP-SERS for breast cancer exosome characterization and detection
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2214180419302272?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subject3-nitrotyrosinept_PT
dc.subjectMolecular imprinted polymerpt_PT
dc.subjectElectrochemical (bio)sensorpt_PT
dc.subjectUrine biomarkerpt_PT
dc.subjectCarbon-printed electrodept_PT
dc.titlePaper-based (bio)sensor for label-free detection of 3-nitrotyrosine in human urine samples using molecular imprinted polymerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleNanobiosensing platform based on MIP-SERS for breast cancer exosome characterization and detection
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FAAG-TEC%2F5400%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FCTM%2F50025%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F94159%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F115173%2F2016/PT
oaire.citation.startPage100333pt_PT
oaire.citation.titleSensing and Bio-Sensing Researchpt_PT
oaire.citation.volume28pt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameMartins
person.familyNameFerreira Sales
person.givenNameGabriela
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-id0219-87C9-5E5B
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0001-6739-1526
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id55918092100
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3a45eaa7-19aa-4321-8ad3-9fc1bb0374d7
relation.isAuthorOfPublicationb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isAuthorOfPublication.latestForDiscovery3a45eaa7-19aa-4321-8ad3-9fc1bb0374d7
relation.isProjectOfPublication33f649e2-6003-4594-9a02-caddafd24896
relation.isProjectOfPublicationfeb26529-3779-47b5-a702-133583fec049
relation.isProjectOfPublication9f27fffb-5536-4654-b7b5-8e47ebc44760
relation.isProjectOfPublicationafbb5d96-5279-4b48-a208-d00ee948b5c7
relation.isProjectOfPublication.latestForDiscoveryfeb26529-3779-47b5-a702-133583fec049

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART_BioMark_Martins_2020.pdf
Size:
1.62 MB
Format:
Adobe Portable Document Format
Download

Collections

ISEP – BioMark – Artigos