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Impact of erythromycin on a non-target organism: Cellular effects on the freshwater microalga Pseudokirchneriella subcapitata

2019Journal article Restricted access
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dc.contributor.authorMachado, Manuela Dias
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2021-03-01T15:54:17Z
dc.date.embargo2120
dc.date.issued2019
dc.description.abstractThe increasing and indiscriminate use of antibiotics is the origin of their introduction in aquatic systems through domestic and livestock effluents. The occurrence of erythromycin (ERY), a macrolide antibiotic, in water bodies raises serious concerns about its potential toxic effect in aquatic biota (non-target organisms), particularly in microalgae, the first organisms in contact with aquatic contaminants. This study aimed to evaluate the possible toxic effects of ERY on relevant cell targets of the freshwater microalga Pseudokirchneriella subcapitata. Algal cells incubated with significant environmental ERY concentrations presented disturbance of the photosynthetic apparatus (increased algal autofluorescence and reduction of chlorophyll a content) and mitochondrial function (hyperpolarization of mitochondrial membrane). These perturbations can apparently be attributed to the similarity of the translational machinery of these organelles (chloroplasts and mitochondria) with the prokaryotic cells. P. subcapitata cells treated with ERY showed a modification of metabolic activity (increased esterase activity) and redox state (alteration of intracellular levels of reactive oxygen species and reduced glutathione content) and an increased biovolume. ERY induced an algistatic effect: reduction of growth rate without loss of cell viability (plasma membrane integrity). The present study shows that chronic exposure (72 h), at low (μg L-1) ERY concentrations (within the range of concentrations detected in surface and ground waters), induce disturbances in the physiological state of the alga P. subcapitata. Additionally, this work alerts to the possible negative impact of the uncontrolled use of ERY on the aquatic systems.pt_PT
dc.description.sponsorshipThis study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/ BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Manuela D.Machado gratefully acknowledges the post-doctoral grant from FCT (SFRH/BPD/72816/2010).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquatox.2019.01.014pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/17205
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-0145-FEDER-006684pt_PT
dc.relationNORTE-01-0145-FEDER-000004pt_PT
dc.relationUSING A FLUORESCENT CELL-BASED APPROACH IN THE EVALUATION OF AQUATIC POLLUTION
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0166445X18309901?via%3Dihub#!pt_PT
dc.subjectCell membrane integrity (viability)pt_PT
dc.subjectErythromycinpt_PT
dc.subjectMetabolic activitypt_PT
dc.subjectMitochondrial functionpt_PT
dc.subjectPhotosynthesispt_PT
dc.subjectToxicitypt_PT
dc.titleImpact of erythromycin on a non-target organism: Cellular effects on the freshwater microalga Pseudokirchneriella subcapitatapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUSING A FLUORESCENT CELL-BASED APPROACH IN THE EVALUATION OF AQUATIC POLLUTION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04469%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F72816%2F2010/PT
oaire.citation.endPage186pt_PT
oaire.citation.startPage179pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume208pt_PT
oaire.fundingStream5876
person.familyNameDias Machado
person.familyNameSoares
person.givenNameManuela
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-id491C-EDE6-F3F3
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-3519-8654
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id23485908300
person.identifier.scopus-author-id7102060173
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscovery98b9a815-89d6-45cf-8d6d-e9de0599a8cd
relation.isProjectOfPublicatione32e2560-63f1-48c6-9d01-9064301c0d5e
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