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ABCC Subfamily Vacuolar Transporters are Involved in Pb (Lead) Detoxification in Saccharomyces cerevisiae

2015Journal article Open access
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dc.contributor.authorSousa, Cátia A.
dc.contributor.authorHanselaer, Simon
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2016-01-15T10:58:48Z
dc.date.available2016-01-15T10:58:48Z
dc.date.issued2015
dc.description.abstractThe present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased susceptibility to high Pb concentrations (500-1000 μmol L(-1)). These results suggest that the formation of glutathione-Pb conjugate (GS-Pb), dependent of GSTs, is important in Pb detoxification. The involvement of ATP-binding cassette (ABC) vacuolar transporters, belonging to class C subfamily (ABCC) in vacuolar compartmentalization of Pb, was evaluated. For this purpose, mutant strains disrupted in YCF1, VMR1, YBT1 or BPT 1 genes were used. All mutants tested, without vacuolar ABCC transporters, presented an increased sensitivity to 500-1000 μmol L(-1) Pb comparative to wild-type strain. Taken together, the obtained results suggest that Pb detoxification, by vacuolar compartmentalization, can occur as a result of the concerted action of GSTs and vacuolar ABCC transporters. Pb is conjugated with glutathione, catalysed by glutathione transferases and followed to the transport of GS-Pb conjugate to the vacuole by ABCC transporters.pt_PT
dc.identifier.doi10.1007/s12010-014-1252-0pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/7405
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation2013 - Strategic Project
dc.relation.ispartofseriesApplied Biochemistry and Biotechnology;Vol. 175, Issue 1
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs12010-014-1252-0pt_PT
dc.subjectGlutathione transferases (GSTs)pt_PT
dc.subjectHeavy metalpt_PT
dc.subjectLead (Pb)pt_PT
dc.subjectVacuolar ABC transporterspt_PT
dc.subjectYeastpt_PT
dc.titleABCC Subfamily Vacuolar Transporters are Involved in Pb (Lead) Detoxification in Saccharomyces cerevisiaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitle2013 - Strategic Project
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.citation.endPage74pt_PT
oaire.citation.startPage65pt_PT
oaire.citation.titleApplied Biochemistry and Biotechnologypt_PT
oaire.citation.volume175pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSoares
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id7102060173
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isProjectOfPublication8a6913fb-272b-4c7d-91e3-7f3a5bdf1f39
relation.isProjectOfPublication.latestForDiscovery8a6913fb-272b-4c7d-91e3-7f3a5bdf1f39

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