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Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches

2021Journal article Open access
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dc.contributor.authorFernandes, Luís
dc.contributor.authorSilva, Hugo
dc.contributor.authorMartins, Inês
dc.contributor.authorCarvalho, Sónia
dc.contributor.authorCarneiro, Isa
dc.contributor.authorHenrique, Rui
dc.contributor.authorTuchin, Valery V.
dc.contributor.authorOliveira, Luís
dc.date.accessioned2022-02-24T12:14:54Z
dc.date.available2022-02-24T12:14:54Z
dc.date.issued2021
dc.description.abstractIn this paper we present three studies that demonstrate the applicability of spectroscopy methods and optical clearing treatments in pathology identification and monitoring. In the first study, by obtaining the absorption spectra of human healthy and pathological (adenocarcinoma) colorectal mucosa tissues, it was possible to identify a higher content of a pigment in the diseased tissues. This study also shows that machine learning methods can be used to reach the same differentiated results in vivo through diffuse reflectance spectroscopy. In the second study, the combination of collimated transmittance spectroscopy with optical clearing treatments allowed to obtain the diffusion coefficients of glucose in healthy and pathological colorectal mucosa as: Dglucose=5.8x10–7 cm2/s and Dglucose=4.4x10–7 cm2/s, respectively. This study also demonstrated that the diseased tissues contains about 5% more mobile water than the healthy tissues. The third study was performed to evaluate the protein dissociation mechanism of optical clearing. By treating both healthy and pathological colorectal mucosa tissues with 93%-glycerol, a protein dissociation rate of about 3 times higher was obtained for the pathological mucosa. All the discriminating parameters that result from these studies can be obtained in the in vivo situation through diffuse reflectance spectroscopy and further studies to evaluate their values in different stages of cancer progression are of great importance to develop disease monitoring protocols.pt_PT
dc.description.sponsorshipThe authors of the article knew well and communicated with Ekaterina Borisova for many years, especially Valery Tuchin and in recent years Luís Oliveira. We had many joint plans to organize conferences and joint research projects, but COVID-19 mercilessly interrupted our communication. We have lost a great scientist and a person with a huge soul, sociable, but at the same time modest and kind. We will always remember our warm meetings and fruitful work with Ekaterina. This research was supported by the Portuguese grant FCT-UIDB/04730/2020. VVT was supported by grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, L., Silva, H., Martins, I., Carvalho, S., Carneiro, I., Henrique, R., Tuchin, V., Oliveira, L. M. (2021). Tissue spectroscopy and optical clearing of colorectal mucosa in the pursuit of new cancer diagnostic approaches. J. Biomed. Phot. & Eng., vol. 7, no. 4, 040302. DOI: 10.18287/JBPE21.07.040302pt_PT
dc.identifier.doi10.18287/JBPE21.07.040302pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/20055
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSamara Universitypt_PT
dc.relationFCT-UIDB/04730/2020pt_PT
dc.relation075-15-2021-615pt_PT
dc.relation.publisherversionhttp://jbpe.ssau.ru/index.php/JBPE/article/view/3447pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectColorectal cancerpt_PT
dc.subjectEnhanced tissue spectroscopypt_PT
dc.subjectCancer detectionpt_PT
dc.subjectOptical clearing mechanismspt_PT
dc.subjectTissue optical clearingpt_PT
dc.titleTissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approachespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage040302-16pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage040302-1pt_PT
oaire.citation.titleJournal of Biomedical Photonics & Engineeringpt_PT
oaire.citation.volume7pt_PT
person.familyNameDias Silva
person.familyNameMartins
person.familyNameOliveira
person.givenNameHugo Filipe
person.givenNameInês
person.givenNameLuís
person.identifier414820
person.identifier.ciencia-idEA11-E3A6-12CB
person.identifier.ciencia-idC312-F342-BD23
person.identifier.orcid0000-0002-6065-4335
person.identifier.orcid0000-0003-2876-3686
person.identifier.orcid0000-0003-0667-3428
person.identifier.ridB-7198-2017
person.identifier.scopus-author-id55853723900
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8e49fbcc-e221-4c7a-aac3-48a2bba5d53a
relation.isAuthorOfPublicationb92cdee2-1e37-46f7-b392-4739075f273d
relation.isAuthorOfPublication4e2de4d7-142a-400f-8dbf-cd0ff9551093
relation.isAuthorOfPublication.latestForDiscovery4e2de4d7-142a-400f-8dbf-cd0ff9551093

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