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Nanocellulose- based biosensor for colorimetric detection of glucose

2020Journal article Open access
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dc.contributor.authorNeubauerova, Katrin
dc.contributor.authorCarneiro, Mariana C.C.G.
dc.contributor.authorRodrigues, Lígia R.
dc.contributor.authorMoreira, Felismina
dc.contributor.authorSales, Maria Goreti Ferreira
dc.date.accessioned2021-09-27T14:21:01Z
dc.date.available2021-09-27T14:21:01Z
dc.date.issued2020
dc.description.abstractThis work reports for the first time a colorimetric based biosensor using nanocellulose (NC) based supports drop-deposited onto a cellulose paper substrate for glucose detection in point-of-care. For this purpose, microcrystalline cellulose (MCC) samples were oxidized with 2,2,6,6-tetramethylpiperidine-N-oxyl radical (TEMPO), sodium hypochlorite, and potassium bromide, to produce carboxylated NC. For the characterization, we used several methods: TEM, FTIR and conductometric titration. In all samples, the primary alcohol groups were selectively oxidized into carboxyl groups, provided the sodium hypochlorite is added dropwise and the reaction is performed at constant pH 10. Carboxyl- NC was further casted on a cellulose substrate and used as support for glucose oxidase (GOx), horseradish peroxidase (HRP) and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) reactions, aiming to yield a coloured detection system for glucose. The sensing system was generated by integrating GOx on the carboxyl- NC /cellulose substrate. Upon reaction with glucose, the enzyme produced hydrogen peroxide, which was converted into a blue-coloured product by reaction with HRP and the chromogenic reagent ABTS. The test-strip was calibrated by incubating it in different concentrations of glucose. The colours obtained were further analysed by a suitable image analysis software. Linear response for glucose ranged from 1.5 to 13.0 mM. Overall, this new test-strip used renewable material for glucose determination, which is an advantage when compared to other systems that require more complex technological approaches. Moreover, it was found that carboxyl- NC improved the colour homogeneity of the test-strip and the intrinsic linear response of concentration range.pt_PT
dc.description.sponsorshipThe Portuguese Science and Technology Foundation, I.P. (FCT), is acknowledged for the financial support through the project reference P2020-PTDC/AAG-TEC/5400/2014 (also POCI-01-0145-FEDER-016637, FEDER, COMPETE, POCI) and POCTEP/INTERREG is also acknowledged through the project 2QBioneuro, Impulso de una red de I+i en química biológica para diagnóstico y tratamiento de enfermedades neurológicas. MCCGC acknowledges FCT for the PhD grant SFRH/BD/131959/2017. The Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.sbsr.2020.100368pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/18574
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationP2020-PTDC/AAG-TEC/5400/2014pt_PT
dc.relationPOCI-01-0145-FEDER-016637pt_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2214180420300970?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectNanocellulosept_PT
dc.subjectTEMPO-based oxidationpt_PT
dc.subjectGlucose oxidasept_PT
dc.subjectColorimetricpt_PT
dc.subjectTest-strippt_PT
dc.subjectBiosensorpt_PT
dc.titleNanocellulose- based biosensor for colorimetric detection of glucosept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.citation.startPage100368pt_PT
oaire.citation.titleSensing and Bio-Sensing Researchpt_PT
oaire.citation.volume29pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMoreira
person.familyNameFerreira Sales
person.givenNameFelismina
person.givenNameMaria Goreti
person.identifier1589429
person.identifier826970
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id23486193000
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isProjectOfPublication.latestForDiscovery222bb02c-0380-41b1-8083-7c6f585e2db3

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