Repository logo
 
Publication

Electrochemical miRNA-34a-based biosensor for the diagnosis of Alzheimer’s disease

2023Journal article Restricted access
Simple item page
dc.contributor.authorPereira, Raquel L.
dc.contributor.authorOliveira, Daniela
dc.contributor.authorPêgo, Ana P.
dc.contributor.authorSantos, Sofia D.
dc.contributor.authorMoreira, Felismina T.C.
dc.date.accessioned2024-04-18T14:23:36Z
dc.date.available2024-04-18T14:23:36Z
dc.date.issued2023
dc.description.abstractAlzheimer's disease (AD) is the most common dementia type and a leading cause of death and disability in the elderly. Diagnosis is expensive and invasive, urging the development of new, affordable, and less invasive diagnostic tools. The identification of changes in the expression of non-coding RNAs prompts the development of diagnostic tools to detect disease-specific blood biomarkers. Building on this idea, this work reports a novel electrochemical microRNA (miRNA) biosensor for the diagnosis of AD, based on carbon screen-printed electrodes (C-SPEs) modified with two gold nanostructures and a complementary anti-miR-34a oligonucleotide probe. This biosensor showed good target affinity, reflected on a 100 pM to 1 μM linearity range and a limit of detection (LOD) of 39 pM in buffer and 94 aM in serum. Moreover, the biosensor’s response was not affected by serum compounds, indicating selectivity for miR-34a. The biosensor also detected miR-34a in the cell culture medium of a common AD model, stimulated with a neurotoxin to increase miR-34a secretion. Overall, the proposed biosensor makes a solid case for the introduction of a novel, inexpensive, and minimally invasive tool for the early diagnosis of AD, based on the detection of a circulating miRNA overexpressed in this pathology.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRaquel L. Pereira, Daniela Oliveira, Ana P. Pêgo, Sofia D. Santos, Felismina T.C. Moreira, Electrochemical miRNA-34a-based biosensor for the diagnosis of Alzheimer’s disease, Bioelectrochemistry, Volume 154, 2023, 108553, ISSN 1567-5394, https://doi.org/10.1016/j.bioelechem.2023.108553.pt_PT
dc.identifier.doi10.1016/j.bioelechem.2023.108553pt_PT
dc.identifier.issn1567-5394
dc.identifier.urihttp://hdl.handle.net/10400.22/25361
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPPBI-POCI-01-0145-FEDER-022122pt_PT
dc.relationUID/EQU/04730/2020pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1567539423001901pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectmiRNA-34apt_PT
dc.subjectElectrochemical biosensorpt_PT
dc.subjectAlzheimer’s diseasept_PT
dc.subjectGold nanoparticlespt_PT
dc.subjectCarbon screen-printed electrodespt_PT
dc.titleElectrochemical miRNA-34a-based biosensor for the diagnosis of Alzheimer’s diseasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBioelectrochemistrypt_PT
oaire.citation.volume154pt_PT
person.familyNamePereira
person.familyNameMoreira
person.givenNameRaquel
person.givenNameFelismina
person.identifier1589429
person.identifier.ciencia-idC917-6A46-A270
person.identifier.orcid0000-0003-0990-4746
person.identifier.orcid0000-0003-4237-8952
person.identifier.scopus-author-id23486193000
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb47ef540-bdc0-497c-92a5-671d2f3801bc
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublication.latestForDiscoveryb47ef540-bdc0-497c-92a5-671d2f3801bc

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
36_Electrochemical miRNA-34a-based biosensor for the diagnosis.pdf
Size:
5.42 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEP – CIETI – Artigos