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Comparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditions

2019Journal article Open access
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dc.contributor.authorFerreira, Carlos M. H.
dc.contributor.authorVilas-Boas, Ângela
dc.contributor.authorSousa, Cátia A.
dc.contributor.authorSoares, Helena M. V. M.
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2021-02-26T11:14:11Z
dc.date.available2021-02-26T11:14:11Z
dc.date.issued2019
dc.description.abstractIron deficiency is one of the main causes of chlorosis in plants, which leads to losses in field crops quality and yield. The use of synthetic chelates to prevent or correct iron-deficiency is not satisfactory mainly due to their poor biodegradability. The present work aimed to search suitable microorganisms to produce alternative, environment-friendly iron-chelating agents (siderophores). For this purpose, the performance of five bacteria (Azotobacter vinelandii, Bacillus megaterium, Bacillus subtilis, Pantoea allii and Rhizobium radiobacter) was evaluated, regarding siderophore production kinetics, level of siderophore production (determined by chrome azurol S, CAS method), type of siderophore produced (using Arnow and Csaky's tests) and iron-chelating capacity at pH 9.0. All bacteria were in stationary phase at 24 h, except A. vinelandii (at 72 h) and produced the maximum siderophore amount (80-140 µmol L-1) between 24 and 48 h, with the exception of A. vinelandii (at 72 h). The analysis of culture filtrates revealed the presence of catechol-type siderophores for B. subtilis and R. radiobacter and hydroxamate-type siderophores for B. megaterium and P. allii. In the case of A. vinelandii, both siderophore-types (catechol and hydroxamates) were detected. The highest iron-chelating capacity, at pH 9.0, was obtained by B. megaterium followed by B. subtilis and A. vinelandii. Therefore, these three bacteria strains are the most promising bacteria for siderophore production and chlorosis correction under alkaline conditions.pt_PT
dc.description.sponsorshipThis work is financed by the FEDER funds through the Operational Competitiveness Factors Program—COMPETE and by national funds through FCT—Foundation for Science and Technology within the scope of the project PTDC-AGR-TEC/0458/2014—POCI-01-0145-FEDER-016681.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1186/s13568-019-0796-3pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/17175
dc.language.isoengpt_PT
dc.publisherSpringerOpenpt_PT
dc.relationPTDC-AGR-TEC/0458/2014—POCI-01-0145-FEDER-016681pt_PT
dc.relation.publisherversionhttps://amb-express.springeropen.com/articles/10.1186/s13568-019-0796-3pt_PT
dc.subjectCatechol and hydroxamates-type siderophorespt_PT
dc.subjectEnvironment-friendly chelating agentspt_PT
dc.subjectIron chelation under alkaline conditionspt_PT
dc.subjectMicroorganismspt_PT
dc.subjectSiderophore productionpt_PT
dc.titleComparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage78pt_PT
oaire.citation.titleAMB Expresspt_PT
oaire.citation.volume9pt_PT
person.familyNameA. Sousa
person.familyNameSoares
person.givenNameCátia
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-id4715-BD01-8460
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-3719-4454
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridS-2889-2016
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id55650545800
person.identifier.scopus-author-id7102060173
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0c464330-a218-49cd-92af-08056285cb74
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscovery0c464330-a218-49cd-92af-08056285cb74

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