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Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

2015Journal article Open access
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dc.contributor.authorMesquita, Vanessa A.
dc.contributor.authorMachado, Manuela D.
dc.contributor.authorSilva, Cristina F.
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2016-01-15T10:30:21Z
dc.date.available2016-01-15T10:30:21Z
dc.date.issued2015
dc.description.abstractMetal contamination of the environment is frequently associated to the presence of two or more metals. This work aimed to study the impact of a mixture of metals (Cd, Pb and Zn) on the physiology of the non-conventional yeast Pichia kudriavzevii. The incubation of yeast cells with 5 mg/l Cd, 10 mg/l Pb and 5 mg/l Zn, for 6 h, induced a loss of metabolic activity (assessed by FUN-1 staining) and proliferation capacity (evaluated by a clonogenic assay), with a small loss of membrane integrity (measured by trypan blue exclusion assay). The staining of yeast cells with calcofluor white revealed that no modification of chitin deposition pattern occurred during the exposure to metal mixture. Extending for 24 h, the exposure of yeast cells to metal mixture provoked a loss of membrane integrity, which was accompanied by the leakage of intracellular components. A marked loss of the metabolic activity and the loss of proliferation capacity were also observed. The analysis of the impact of a single metal has shown that, under the conditions studied, Pb was the metal responsible for the toxic effect observed in the metal mixture. Intracellular accumulation of Pb seems to be correlated with the metals' toxic effects observed.pt_PT
dc.identifier.doi10.1007/s11356-015-4326-9pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/7401
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationPEst- OE/EQB/LA0023/2013pt_PT
dc.relationNORTE- 07-0124-FEDER-000028pt_PT
dc.relation.ispartofseriesEnvironmental Science and Pollution Research;Vol. 22
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs11356-015-4326-9pt_PT
dc.subjectCell deadpt_PT
dc.subjectHeavy metalspt_PT
dc.subjectMembrane integritypt_PT
dc.subjectMetabolic activity inhibitionpt_PT
dc.subjectMetals toxicitypt_PT
dc.subjectYeast survivalpt_PT
dc.titleImpact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzeviipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage11136pt_PT
oaire.citation.startPage11127pt_PT
oaire.citation.titleEnvironmental Science and Pollution Researchpt_PT
oaire.citation.volume22pt_PT
person.familyNameSoares
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id7102060173
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219

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