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Palmelloid-like phenotype in the alga Raphidocelis subcapitata exposed to pollutants: A generalized adaptive strategy to stress or a specific cellular response?

2023Journal article Restricted access
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dc.contributor.authorMachado, Manuela D.
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2024-04-18T14:24:42Z
dc.date.available2024-04-18T14:24:42Z
dc.date.issued2023
dc.description.abstractThis work focuses on the formation of palmelloid-like phenotype in the freshwater alga Raphidocelis subcapitata (formerly known as Pseudokirchneriella subcapitata and Selenastrum capricornutum), when exposed to adverse conditions generated by the presence of organic [the antibiotic erythromycin (ERY) and the herbicide metolachlor (MET)] or inorganic [the heavy metals, cadmium (Cd) and zinc (Zn)] pollutants, at environmentally relevant concentrations. This alga in absence of stress or when exposed to ERY or Zn, up to 200 µg/L, essentially showed a single-nucleus state, although algal growth was reduced or stopped. R. subcapitata “switched” to a multinucleated state (palmelloid-like morphology) and accumulated energy-reserve compounds (neutral lipids) when stressed by 100–200 µg/L MET or 200 µg/L Cd; at these concentrations of pollutants, growth was arrested, however, the majority of the algal population (≥83 %) was alive. The formation of palmelloid-like phenotype, at sub-lethal concentrations of pollutants, was dependent on the pollutant, its concentration and exposure time. The multinucleated structure is a transitory phenotype since R. subcapitata population was able to revert to a single-nucleus state, with normal cell size, within 24–96 h (depending on the impact of the toxic in the alga), after being transferred to fresh OECD medium, without pollutants. The obtained results indicate that the formation of a palmelloid-like phenotype in R. subcapitata is dependent on the mode of action of toxics and their concentration, not constituting a generalized defense mechanism against stress. The observations here shown contribute to understanding the different strategies used by the unicellular alga R. subcapitata to cope with severe stress imposed by organic and inorganic pollutants.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationManuela D. Machado, Eduardo V. Soares, Palmelloid-like phenotype in the alga Raphidocelis subcapitata exposed to pollutants: A generalized adaptive strategy to stress or a specific cellular response?, Aquatic Toxicology, Volume 264, 2023, 106732, ISSN 0166-445X, https://doi.org/10.1016/j.aquatox.2023.106732.pt_PT
dc.identifier.doi10.1016/j.aquatox.2023.106732pt_PT
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10400.22/25363
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationDL 57/2016pt_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.relationAssociate Laboratory on Biotechnology, Bioengineering and microELectromechanical Systems
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0166445X2300334Xpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAsexual reproductionpt_PT
dc.subjectErythromycinpt_PT
dc.subjectHeavy metalspt_PT
dc.subjectMetolachlorpt_PT
dc.subjectPalmelloid phenotypept_PT
dc.subjectStress responsept_PT
dc.titlePalmelloid-like phenotype in the alga Raphidocelis subcapitata exposed to pollutants: A generalized adaptive strategy to stress or a specific cellular response?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardTitleAssociate Laboratory on Biotechnology, Bioengineering and microELectromechanical Systems
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0029%2F2020/PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume264pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDias Machado
person.familyNameSoares
person.givenNameManuela
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-id491C-EDE6-F3F3
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-3519-8654
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id23485908300
person.identifier.scopus-author-id7102060173
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscovery98b9a815-89d6-45cf-8d6d-e9de0599a8cd
relation.isProjectOfPublication222bb02c-0380-41b1-8083-7c6f585e2db3
relation.isProjectOfPublication9437ec14-7cbb-4c5e-abb2-41e299169b5e
relation.isProjectOfPublication.latestForDiscovery9437ec14-7cbb-4c5e-abb2-41e299169b5e

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