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Desenvolvimento de um sensor ótico biomimético para a deteção da proteína CA19-9
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Abstract(s)
A doença oncológica, nas últimas décadas, tem demonstrado uma tendência crescente,
tornando-se num problema de saúde pública. Um dos pontos principais para um melhor
prognóstico consiste na deteção precoce do cancro, contudo quando estes são assintomáticos,
como o cancro do pâncreas, a deteção, muitas vezes, é tardia, deixando passar uma
oportunidade para tratamento que seria importante. Esta deteção tardia traduz-se na alta taxa
de mortalidade dos pacientes com cancro do pâncreas. Para colmatar esta lacuna foram
desenvolvidas novas técnicas de deteção, como o desenvolvimento de sensores biomiméticos
para biomarcadores, como o antigénio de carboidrato 19-9 (CA19-9, do inglês, Carbohydrate
antigen 19-9).
O sensor biomimético foi criado pela técnica de impressão de superfície, criando um polímero
molecularmente impresso (MIPs, do inglês, molecularly imprinted polymers), associado a
pontos quânticos (QDs, do inglês, quantum dots) (MIP@QDs).
O sensor desenvolvido ao longo deste projeto demonstrou sensibilidade e especificidade na
deteção do biomarcador do cancro do pâncreas, CA19-9, em solução. Perante a boa resposta
do sensor em solução, durante este trabalho, foi realizado, ainda, um estudo em suporte sólido,
em que também mostrou uma boa resposta aquando da presença do analito. Os estudos em
membrana, visam testar a produção de um sensor que possa ser portátil, eficaz e sensível para
chegar às diversas regiões do mundo, permitindo o acesso à saúde, promovendo a deteção
precoce e um tratamento mais eficaz.
Over the past few decades, cancer has been on the rise, becoming a public health problem. One of the main points for a better prognosis is the early detection of cancer, but when cancers are asymptomatic, such as pancreatic cancer, detection is often late, missing an important opportunity for treatment. This late detection is reflected in the high mortality rate of pancreatic cancer patients. To bridge this gap, new detection techniques have been developed, such as the development of biomimetic sensors for biomarkers such as carbohydrate antigen 19-9 (CA19-9). The biomimetic sensor was created using the surface imprinting technique, creating a molecularly imprinted polymer (MIPs) associated with quantum dots (QDs) (MIP@QDs). The sensor developed during this project showed sensitivity and specificity in detecting the pancreatic cancer biomarker CA19-9 in solution. Given the good response of the sensor in solution, during this work, a study was also carried out on solid support, which also showed a good response in the presence of the analyte. The membrane studies aim to test the production of a sensor that can be portable, effective and sensitive so that it can reach different regions of the world, allowing access to health, promoting early detection and more effective treatment.
Over the past few decades, cancer has been on the rise, becoming a public health problem. One of the main points for a better prognosis is the early detection of cancer, but when cancers are asymptomatic, such as pancreatic cancer, detection is often late, missing an important opportunity for treatment. This late detection is reflected in the high mortality rate of pancreatic cancer patients. To bridge this gap, new detection techniques have been developed, such as the development of biomimetic sensors for biomarkers such as carbohydrate antigen 19-9 (CA19-9). The biomimetic sensor was created using the surface imprinting technique, creating a molecularly imprinted polymer (MIPs) associated with quantum dots (QDs) (MIP@QDs). The sensor developed during this project showed sensitivity and specificity in detecting the pancreatic cancer biomarker CA19-9 in solution. Given the good response of the sensor in solution, during this work, a study was also carried out on solid support, which also showed a good response in the presence of the analyte. The membrane studies aim to test the production of a sensor that can be portable, effective and sensitive so that it can reach different regions of the world, allowing access to health, promoting early detection and more effective treatment.
Description
Keywords
Pancreatic cancer CA19-9 protein biomimetic sensor surface imprinting MIP@QDs Cancro do pâncreas Proteína CA19-9 Impressão de superfície sensor biomimético