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Nickel Oxide Nanoparticles Trigger Caspase- and Mitochondria-Dependent Apoptosis in the Yeast Saccharomyces cerevisiae

2019Journal article Restricted access
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dc.contributor.authorSousa, Cátia A.
dc.contributor.authorSoares, Helena M. V. M.
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2021-03-01T15:32:05Z
dc.date.embargo2120
dc.date.issued2019
dc.description.abstractThe expansion of the industrial use of nickel oxide (NiO) nanoparticles (NPs) raises concerns about their potential adverse effects. Our work aimed to investigate the mechanisms of toxicity induced by NiO NPs, using the yeast Saccharomyces cerevisiae as a cell model. Yeast cells exposed to NiO NPs exhibited typical hallmarks of regulated cell death (RCD) by apoptosis [loss of cell proliferation capacity (cell viability), exposure of phosphatidylserine at the outer cytoplasmic membrane leaflet, nuclear chromatin condensation, and DNA damage] in a process that required de novo protein synthesis. The execution of yeast cell death induced by NiO NPs is Yca1p metacaspase-dependent. NiO NPs also induced a decrease in the mitochondrial membrane potential and an increase in the frequency of respiratory-deficient mutants, which supports the involvement of mitochondria in the cell death process. Cells deficient in the apoptosis-inducing factor ( aif1Δ) displayed higher tolerance to NiO NPs, which reinforces the involvement of mitochondria in RCD by apoptosis. In summary, this study shows that NiO NPs induce caspase- and mitochondria-dependent apoptosis in yeast. Our results warn about the possible harmful effects associated with the use of NiO NPs.pt_PT
dc.description.sponsorshipThis work was performed in the framework of the financing by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684), and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020Programa Operacional Regional do Norte and LAQV (UID/QUI/50006/2013-POCI/01/0145/FEDER/007265) with financial support from FCT/MEC through national funds and cofinanced by FEDER, under Partnership Agreement PT2020. C.A.S. gratefully acknowledges the doctoral grant (SFRH/BD/101452/2014) from FCT.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1021/acs.chemrestox.8b00265pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/17203
dc.language.isoengpt_PT
dc.publisherACS Publications
dc.relationPOCI-01-0145-FEDER-006684pt_PT
dc.relationNORTE-01-0145-FEDER-000004pt_PT
dc.relationUID/QUI/50006/2013-POCI/01/0145/FEDER/007265pt_PT
dc.relationImpact of nanoparticles in fresh water aquatic systems: physico-chemical characteristics versus ecotoxicological effects
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acs.chemrestox.8b00265
dc.subjectApoptosispt_PT
dc.subjectCaspasespt_PT
dc.subjectDNA Damagept_PT
dc.subjectMembrane Potential, Mitochondrialpt_PT
dc.subjectMitochondriapt_PT
dc.subjectNADH, NADPH Oxidoreductasespt_PT
dc.subjectNanoparticlespt_PT
dc.subjectNickelpt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectSaccharomyces cerevisiae Proteinspt_PT
dc.titleNickel Oxide Nanoparticles Trigger Caspase- and Mitochondria-Dependent Apoptosis in the Yeast Saccharomyces cerevisiaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImpact of nanoparticles in fresh water aquatic systems: physico-chemical characteristics versus ecotoxicological effects
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04469%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F101452%2F2014/PT
oaire.citation.endPage254pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage245pt_PT
oaire.citation.titleChemical Research in Toxicologypt_PT
oaire.citation.volume32pt_PT
oaire.fundingStream5876
person.familyNameA. Sousa
person.familyNameSoares
person.givenNameCátia
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-id4715-BD01-8460
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-3719-4454
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridS-2889-2016
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id55650545800
person.identifier.scopus-author-id7102060173
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0c464330-a218-49cd-92af-08056285cb74
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscovery0c464330-a218-49cd-92af-08056285cb74
relation.isProjectOfPublicatione32e2560-63f1-48c6-9d01-9064301c0d5e
relation.isProjectOfPublication7fc14083-aac2-4d16-9ac2-4f4dadc0cc2c
relation.isProjectOfPublication.latestForDiscoverye32e2560-63f1-48c6-9d01-9064301c0d5e

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