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Electrochemistry-assisted surface plasmon resonance detection of miRNA-145 at femtomolar level

2020Journal article Open access
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dc.contributor.authorRibeiro, José A.
dc.contributor.authorSales, Maria Goreti Ferreira
dc.contributor.authorPereira, Carlos M.
dc.date.accessioned2021-09-27T14:14:37Z
dc.date.available2021-09-27T14:14:37Z
dc.date.issued2020
dc.description.abstractIn this work, we combined electrochemical techniques with SPR (eSPR) for the label-free detection of cancer biomarker miRNA-145. Detection was performed in a simple two-step assay. In the first step, the gold sensor surface, previously functionalized with a self-assembled monolayer (SAM) of thiolated RNA probes is incubated with the sample containing the target RNA biomarker. In this step, hybridization of RNA fragments with complementary immobilized probes was monitored in real-time by SPR. In the second step, eSPR measurements were performed to improve the sensitivity of the hybridization assay. Potential-induced deposition of a redox probe at the sensor surface resulted in enhanced SPR response promoted by the electrochemical process, thereby allowing the detection of miRNA-145 at femtomolar level (LOD = 0.56 fM), without sample derivatization or post-hybridization treatment for signal amplification. Good linearity was achieved (R2 = 0.984) over the concentration range from 1.0 fM and 10 nM. Furthermore, the developed eSPR biosensor showed high selectivity towards single-base and two-base mismatch sequences and detection of target miRNA-145 in synthetic human serum was successful achieved.pt_PT
dc.description.sponsorshipThis research had the financial support of FCT (Fundação para a Ciência e Tecnologia) and co-financed by the European Union (FEDER funds) under the Partnership Agreement PT2020, Research Grants UIDB/00081/2020 (CIQUP), NORTE-2020 under the project RamSERS (grant NORTE-01-0247-FEDER-017834). J.A. Ribeiro (ref. SFRH/BPD/105395/2014) acknowledges FCT under the QREN – POPH – Advanced Training, subsidized by European Union and national MEC funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2020.128129pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/18572
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-01-0247-FEDER-017834pt_PT
dc.relationChemistry Research Unit of University of Porto
dc.relationNanomateriais aplicados à bideteção eletroquímica e ótica de biomarcadores do cancro
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0925400520304792?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBiomarkerpt_PT
dc.subjectBiosensorpt_PT
dc.subjectElectrochemistrypt_PT
dc.subjectmiRNA-145pt_PT
dc.subjectSurface plasmon resonance (SPR)pt_PT
dc.titleElectrochemistry-assisted surface plasmon resonance detection of miRNA-145 at femtomolar levelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleChemistry Research Unit of University of Porto
oaire.awardTitleNanomateriais aplicados à bideteção eletroquímica e ótica de biomarcadores do cancro
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00081%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F105395%2F2014/PT
oaire.citation.startPage128129pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume316pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamFARH
person.familyNameFerreira Sales
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isProjectOfPublication2a43ea7f-a750-492c-bafc-d818da22f041
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