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Development of a novel electrochemical biosensor based on plastic antibodies for detection of STEAP1 biomarker in cancer

2023Journal article Restricted access
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dc.contributor.authorCarvalho, Margarida
dc.contributor.authorRocha, Sandra Moreira
dc.contributor.authorBarroca-Ferreira, Jorge
dc.contributor.authorMaia, Claudio J.
dc.contributor.authorGuillade, Lucía
dc.contributor.authorCorrea-Duarte, Miguel A.
dc.contributor.authorPassarinha, Luís A.
dc.contributor.authorMoreira, Felismina T.C.
dc.contributor.authorGomes, Rui M.
dc.date.accessioned2024-04-18T14:23:07Z
dc.date.available2024-04-18T14:23:07Z
dc.date.issued2023
dc.description.abstractSTEAP1 is a cell surface protein of the STEAP family whose main function focuses on intercellular communication and cell growth. STEAP1 is considered a promising putative biomarker and a candidate target for prostate cancer treatment. For specific and selective detection of STEAP1, a molecularly imprinted polymers (MIP) was developed on a screen-printed electrode (C-SPE) whose surface was modified with a nanocomposite based on carbon nanotubes decorated with dendritic platinum nanoparticles (CNTs- PAH /Pt). Then, the MIPs were produced on the modified C-SPE by electropolymerization of a mixture of STEAP1 and a monomer (pyrrole-2-carboxylic acid). Then, the protein was removed from the polymeric network by enzymatic treatment with trypsin, which created the specific template cavities for further STEAP1 detection. Electrochemical techniques such as EIS and CV were used to follow the chemical modification steps of C-SPE. The analytical performance of the biosensor was evaluated by SWV in PBS buffer and in lysates of neoplastic prostate cancer cells (LNCaP) extracts. The MIP material showing a linear range from 130 pg/ml to 13 µg/ml. Overall, the biosensor exhibits essential properties such as selectivity, sensitivity and reproducibility for its application in medical and clinical research diagnosis and/or prognosis of prostate cancer.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMargarida Carvalho, Rui M. Gomes, Sandra Moreira Rocha, Jorge Barroca-Ferreira, Claudio J. Maia, Lucía Guillade, Miguel A. Correa-Duarte, Luís A. Passarinha, Felismina T.C. Moreira, Development of a novel electrochemical biosensor based on plastic antibodies for detection of STEAP1 biomarker in cancer, Bioelectrochemistry, Volume 152, 2023, 108461, ISSN 1567-5394, https://doi.org/10.1016/j.bioelechem.2023.108461.pt_PT
dc.identifier.doi10.1016/j.bioelechem.2023.108461pt_PT
dc.identifier.issn1567-5394
dc.identifier.urihttp://hdl.handle.net/10400.22/25360
dc.language.isospapt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1567539423000981pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectProstate cancerpt_PT
dc.subjectBiosensorpt_PT
dc.subjectSTEAP1pt_PT
dc.subjectMolecularly imprinted polymerspt_PT
dc.titleDevelopment of a novel electrochemical biosensor based on plastic antibodies for detection of STEAP1 biomarker in cancerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBioelectrochemistrypt_PT
oaire.citation.volume152pt_PT
person.familyNameMoreira Rocha
person.familyNameCorrea-Duarte
person.familyNamePassarinha
person.familyNameMoreira
person.givenNameSandra
person.givenNameMiguel A.
person.givenNameLuís
person.givenNameFelismina
person.identifierE-3127-2015
person.identifier81160
person.identifier131188
person.identifier1589429
person.identifier.ciencia-id7C1B-D6AB-9A28
person.identifier.ciencia-id531D-D97E-5153
person.identifier.ciencia-id621B-74BF-F9A7
person.identifier.ciencia-idC917-6A46-A270
person.identifier.orcid0000-0002-4686-2410
person.identifier.orcid0000-0003-1950-1414
person.identifier.orcid0000-0001-6910-7576
person.identifier.orcid0000-0003-4237-8952
person.identifier.scopus-author-id23488450500
person.identifier.scopus-author-id12445439600
person.identifier.scopus-author-id23486193000
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa4c236c6-4e5b-45aa-8538-c87c2b16360a
relation.isAuthorOfPublication540d135d-7e45-4f17-a975-0820805046f3
relation.isAuthorOfPublicationa55631f0-bbd4-4339-99c5-aed4a009753f
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublication.latestForDiscovery9c13153d-a73a-49ff-b7f6-c9733bc6725e

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